GloCell™ Fixable Viability Dye UV 450
Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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In-lab demonstration
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D-PBS (Without Ca++ and Mg++)Dulbecco’s phosphate-buffered saline without calcium and magnesium
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EasySep™ BufferCell separation buffer
Overview
GloCell™ Fixable Viability Dye UV 450 is excited by the UV laser at 355 nm and it has a fluorescence emission maximum of 450 nm that can be detected using the 450/50 band pass filter used to detect DAPI, Hoechst 33258, etc.
Data Figures
Figure 1.
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye UV 450. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye UV 450.
Figure 2.
Excitation/Emission spectra for GloCell™ Fixable Viability Dye UV 450. Excitation max: 355 nm, Emission Max: 450 nm
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
