GloCell™ Fixable Viability Dye UV 450
Amine-labeling fluorescent dye for live/dead staining of mammalian cells
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GloCell™ Fixable Viability Dye UV 450 -
Label for GloCell™ Fixable Viability Dye UV 450
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Required Products
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Cell separation buffer -
Dulbecco’s phosphate-buffered saline without calcium and magnesium
Overview
GloCell™ Fixable Viability Dye UV 450 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.
GloCell™ Fixable Viability Dye UV 450 is excited by the UV laser at 355 nm and it has a fluorescence emission maximum of 450 nm that can be detected using the 450/50 band pass filter used to detect DAPI, Hoechst 33258, etc.
GloCell™ Fixable Viability Dye UV 450 is excited by the UV laser at 355 nm and it has a fluorescence emission maximum of 450 nm that can be detected using the 450/50 band pass filter used to detect DAPI, Hoechst 33258, etc.
Application:
Flow Cytometry; Immunofluorescence
Brand:
GloCell
Area of Interest:
Angiogenic Cell Research; Antibody Development; Cancer Research; Cell Line Development; Chimerism; Disease Modeling; Drug Discovery and Toxicity Testing; Endothelial Cell Biology; Epithelial Cell Biology; HIV; HLA; Hybridoma Generation; Immunology; Infectious Diseases; Metabolism; Mitosis; Neuroscience; Organoids; Respiratory Research; Stem Cell Biology; Cell Therapy; Transplantation Research
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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Figure 1.
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye UV 450. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye UV 450.
Figure 2.
Excitation/Emission spectra for GloCell™ Fixable Viability Dye UV 450. Excitation max: 355 nm, Emission Max: 450 nm
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.