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Figure 1. Staining Profiles of Various Cell Populations
(A) E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit on poly-lysine-coated glass coverslips, then fixed and labeled with Anti-Beta-Tubulin III Antibody, Clone AA10, Alexa Fluor® 488 (Catalog #60100AD, green), and counterstained with DAPI (blue). Inset shows cells incubated with a mouse IgG2a, kappa isotype control antibody, Alexa Fluor® 488, and counterstained with DAPI. (B) Imaging flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, PE (red, Catalog #60018PE) and counterstained with DAPI (blue). Staining of a non-viable leukocyte is shown in the top panel and staining of a viable leukocyte in the bottom panel. (C) Chemical structure of DAPI (Hydrochloride).
Protocols and Documentation
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.