Human Recombinant FGF-10 (KGF-2), ACF

Fibroblast growth factor 10, animal component-free

Human Recombinant FGF-10 (KGF-2), ACF

Fibroblast growth factor 10, animal component-free

From: 672 USD
Catalog #
78173_C
Fibroblast growth factor 10, animal component-free

Overview

Fibroblast growth factor 10 (FGF-10) is a member of the fibroblast growth factor (FGF) family which is predominantly expressed by mesenchymal fibroblasts during embryonic development (Emoto et al.; Igarashi et al.). It binds with high affinity to fibroblast growth factor receptor 2-IIIb (FGFR2-IIIb), and also has a weaker affinity for FGFR1-IIIb (Beer et al.). FGF-10 and FGF-7 have similar receptor binding properties and target cell specificities but are differentially regulated by components of the extracellular matrix (Emoto et al.; Igarashi et al.). FGF-10 has been shown to mediate epithelial-mesenchymal interactions, which are essential to lung development (Sekine et al.; Ware & Matthay). FGF-10 also has a role in mobilization and proliferation of lung-resident mesenchymal stem cells (MSCs) and protection and repair against acute lung injury (Tong et al.; Ware & Matthay) and endodermal differentiation of human pluripotent stem cells to insulin-producing pancreatic-like cells (Takeuchi et al.). This product is animal component-free.
Subtype
Cytokines, Growth Factors
Alternative Names
Fibroblast growth factor 10, Keratinocyte growth factor 2
Cell Type
Endoderm, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Keratinocytes, Mesenchymal Stem and Progenitor Cells, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells, Prostate Cells
Species
Human
Area of Interest
Epithelial Cell Biology, Neuroscience, Stem Cell Biology
Molecular Weight
19.3 kDa
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant FGF-10 (KGF-2), ACF was tested by its ability to promote proliferation of 4MBr-5 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 6.45 ng/mL. (B) 1 μg of Human Recombinant FGF-10 (KGF-2), ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant FGF-10 (KGF-2), ACF has a predicted molecular mass of 19.3 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78173.1, 78173.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78173.1, 78173.2
Lot #
All
Language
English

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