STEMdiff™ Lung Progenitor Kit

Serum-free medium for efficient and reproducible generation of lung progenitor cells from human ES and iPS cells
STEMdiff™ Lung Progenitor Kit

Serum-free medium for efficient and reproducible generation of lung progenitor cells from human ES and iPS cells

1 Kit
Catalog # 100-0230
891 EUR
Required Products
  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. Y-27632 (Dihydrochloride)
    Y-27632

    RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2

  3. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

  4. mTeSR™1
    mTeSR™1

    cGMP, feeder-free maintenance medium for human ES and iPS cells

Overview

STEMdiff™ Lung Progenitor Kit is a serum-free culture medium system for efficient and reproducible generation of lung progenitor cells from human embryonic stem (ES) and induced pluripotent stem (iPS) cells through three stages of differentiation: 1) definitive endoderm, 2) anterior foregut endoderm, and 3) lung progenitor cells. Differentiated cells will express NKX2.1, a key marker of lung progenitor cells. The resulting cells can be further matured towards proximal or distal airway cells using published protocols for the study of lung diseases and lung development.
Advantages
⦁ Differentiate hPSCs to lung progenitor cells with a robust medium, optimized to work with mTeSR™1
⦁ Obtain lung progenitor cells capable of downstream differentiation to proximal and distal airway cells
⦁ Maximize experimental reproducibility with an optimized, serum-free formulation
⦁ Convenient format and easy-to-use protocol
Components
  • STEMdiff™ Endoderm Basal Medium, 100 mL
  • STEMdiff™ Definitive Endoderm Supplement MR (100X), 0.35 mL
  • STEMdiff™ Definitive Endoderm Supplement CJ (100X), 1.1 mL
  • STEMdiff™ Lung Basal Medium, 180 mL
  • STEMdiff™ Lung Supplement (10X), 20 mL
  • STEMdiff™ Lung Progenitor Supplement 1 (100X), 0.55 mL
  • STEMdiff™ Lung Progenitor Supplement 2 (100X), 1.45 mL
Subtype
Specialized Media
Cell Type
Airway Cells, Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Differentiation, Organoid Culture
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Respiratory Research, Stem Cell Biology
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Lung Progenitor Kit
Catalog #
100-0230
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Lung Progenitor Kit
Catalog #
100-0230
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Lung Progenitor Kit
Catalog #
100-0230
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
STEMdiff™ Lung Progenitor Kit
Catalog #
100-0230
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
STEMdiff™ Lung Progenitor Kit
Catalog #
100-0230
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
STEMdiff™ Lung Progenitor Kit
Catalog #
100-0230
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
STEMdiff™ Lung Progenitor Kit
Catalog #
100-0230
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
STEMdiff™ Lung Progenitor Kit
Catalog #
100-0230
Lot #
All
Language
English

Educational Materials (9)

Brochure
STEMdiff™ Definitive Endoderm Kit
Brochure
Products for Human Pluripotent Stem Cells
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Cellular Organization and Biology of the Respiratory System
Webinar
Making Lung Cells from Pluripotent Stem Cells: Disease Modeling and Future Therapies
44:40
Making Lung Cells from Pluripotent Stem Cells: Disease Modeling and Future Therapies
Webinar
STEMdiff™ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
20:24
STEMdiff™ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
Webinar
hPSC-Derived Lung Models as Tools for Respiratory Research
17:17
hPSC-Derived Lung Models as Tools for Respiratory Research
Scientific Poster
Efficient Generation of Lung Progenitor Cells From Human Pluripotent Stem Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Illustration showing the protocol, media, and three stages (definitive endoderm, anterior foregut endoderm, and lung progenitors) invoved in the differentiation of human PSCs into lung progenitor cells using the STEMdiff™ Lung Progenitor Kit.

Figure 1. Generation of Lung Progenitor Cells from Human ES/iPS Cells with the STEMdiff™ Lung Progenitor Kit

Human PSC (hPSC) cultures progress through a simple three-stage process to generate lung progenitor cells. hPSC clumps are first seeded in mTeSR™1. On Day 1, differentiation is initiated with Medium DE-1 (STEMdiff™ Endoderm Basal Medium containing Supplement MR and Supplement CJ). Subsequently on day 2 and 3, the medium is changed to Medium DE-2 (STEMdiff™ Endoderm Basal Medium containing Supplement CJ) for definitive endoderm patterning. On Day 4, to initiate anterior foregut endoderm patterning, the endoderm monolayer is passaged in Medium LP-1 (STEMdiff™ Lung Basal Medium, Lung Supplement (10X), and Supplement 1) and Y-27632. Finally, at Day 7, the cells are differentiated into the lung progenitor stage with Medium LP-2 (STEMdiff™ Lung Basal Medium, Lung Supplement (10X), and Supplement 2).

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