STEMdiff™ Lung Progenitor Kit
Serum-free medium for efficient and reproducible generation of lung progenitor cells from human ES and iPS cells
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STEMdiff™ Lung Progenitor Kit -
STEMdiff™ Endoderm Basal Medium (Lung) -
STEMdiff™ Definitive Endoderm Supplement MR (100X) -
STEMdiff™ Definitive Endoderm Supplement CJ (100X) -
STEMdiff™ Lung Basal Medium -
STEMdiff™ Lung Supplement (10X) -
STEMdiff™ Lung Progenitor Supplement 1 (100X) -
STEMdiff™ Lung Progenitor Supplement 2 (100X)
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Required Products
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cGMP, feeder-free maintenance medium for human ES and iPS cells -
Gentle Cell Dissociation Reagent
Enzyme-free cell dissociation reagent -
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2 -
Dulbecco’s phosphate-buffered saline without calcium and magnesium
Overview
STEMdiff™ Lung Progenitor Kit is a serum-free culture medium system for efficient and reproducible generation of lung progenitor cells from human embryonic stem (ES) and induced pluripotent stem (iPS) cells through three stages of differentiation: 1) definitive endoderm, 2) anterior foregut endoderm, and 3) lung progenitor cells. Differentiated cells will express NKX2.1, a key marker of lung progenitor cells. The resulting cells can be further matured towards proximal or distal airway cells using published protocols for the study of lung diseases and lung development.
Advantages:
⦁ Differentiate hPSCs to lung progenitor cells with a robust medium, optimized to work with mTeSR™1
⦁ Obtain lung progenitor cells capable of downstream differentiation to proximal and distal airway cells
⦁ Maximize experimental reproducibility with an optimized, serum-free formulation
⦁ Convenient format and easy-to-use protocol
⦁ Obtain lung progenitor cells capable of downstream differentiation to proximal and distal airway cells
⦁ Maximize experimental reproducibility with an optimized, serum-free formulation
⦁ Convenient format and easy-to-use protocol
Components:
- STEMdiff™ Endoderm Basal Medium, 100 mL
- STEMdiff™ Definitive Endoderm Supplement MR (100X), 0.35 mL
- STEMdiff™ Definitive Endoderm Supplement CJ (100X), 1.1 mL
- STEMdiff™ Lung Basal Medium, 180 mL
- STEMdiff™ Lung Supplement (10X), 20 mL
- STEMdiff™ Lung Progenitor Supplement 1 (100X), 0.55 mL
- STEMdiff™ Lung Progenitor Supplement 2 (100X), 1.45 mL
Subtype:
Specialized Media
Cell Type:
Airway Cells; Pluripotent Stem Cells
Species:
Human
Application:
Cell Culture; Differentiation; Organoid Culture
Brand:
STEMdiff
Area of Interest:
Disease Modeling; Drug Discovery and Toxicity Testing; Epithelial Cell Biology; Respiratory Research; Stem Cell Biology
Formulation:
Serum-Free
Related Products
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STEMdiff™ Definitive Endoderm Kit
Defined animal component-free medium for the differentiation of human ES and iPS cells to definitive endoderm -
Serum- and BPE-free medium for human small airway epithelial cells cultured at the air-liquid interface -
Serum- and BPE-free medium for human airway epithelial cells cultured at the air-liquid interface or as airway organoids -
PneumaCult™ Airway Organoid Kit
Serum- and BPE-free medium for efficient establishment and differentiation of airway organoids
Scientific Resources
Product Documentation
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Data
Figure 1. Generation of Lung Progenitor Cells from Human ES/iPS Cells with the STEMdiff™ Lung Progenitor Kit
Human PSC (hPSC) cultures progress through a simple three-stage process to generate lung progenitor cells. hPSC clumps are first seeded in mTeSR™1. On Day 1, differentiation is initiated with Medium DE-1 (STEMdiff™ Endoderm Basal Medium containing Supplement MR and Supplement CJ). Subsequently on day 2 and 3, the medium is changed to Medium DE-2 (STEMdiff™ Endoderm Basal Medium containing Supplement CJ) for definitive endoderm patterning. On Day 4, to initiate anterior foregut endoderm patterning, the endoderm monolayer is passaged in Medium LP-1 (STEMdiff™ Lung Basal Medium, Lung Supplement (10X), and Supplement 1) and Y-27832. Finally, at Day 9, the cells are differentiated into the lung progenitor stage with Medium LP-2 (STEMdiff™ Lung Basal Medium, Lung Supplement (10X), and Supplement 2).
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.