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PneumaCult™ Airway Organoid Kit

Serum- and BPE-free medium for efficient establishment and differentiation of airway organoids

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Interested in trying STEMCELL's organoid products for respiratory research? Fill out this form to request information about introductory offers.

PneumaCult™ Airway Organoid Kit

Serum- and BPE-free medium for efficient establishment and differentiation of airway organoids

From: 963 USD
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Serum- and BPE-free medium for efficient establishment and differentiation of airway organoids
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Product Advantages


  • Three-dimensional in vitro system that recapitulates key characteristics of the in vivo human airway

  • Complete media formulation for expansion and differentiation of human airway epithelial cells, compatible with PneumaCult™-Ex Plus

  • Convenient format and easy-to-use protocol that delivers consistent results

What's Included

  • PneumaCult™ Airway Organoid Basal Medium, 450 mL (Catalog #05061)
  • PneumaCult™ Airway Organoid Seeding Supplement, 10 mL (Catalog #05062)
  • PneumaCult™ Airway Organoid Differentiation Supplement, 40 mL (Catalog #05063)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. PneumaCult™-Ex Plus Medium
    PneumaCult™-Ex Plus Medium

    Serum- and BPE-free medium for expansion of primary human airway epithelial cells

  2. Animal Component-Free Cell Dissociation Kit
    Animal Component-Free Cell Dissociation Kit

    Dissociation kit for human stem and progenitor cells

  3. Hydrocortisone Stock Solution|07925
    Hydrocortisone Stock Solution

    Cell culture supplement

  4. Heparin Solution
    Heparin Solution

    Cell culture supplement

Overview

PneumaCult™ Airway Organoid Kit is a serum- and BPE-free culture medium system for efficient establishment and differentiation of airway organoids derived from human bronchial epithelial cells (HBECs).
After 2D expansion in PneumaCult™-Ex Plus Medium (#05040), PneumaCult™ Airway Organoid Seeding Medium allows for initiation of 3D organoid culture, then PneumaCult™ Airway Organoid Differentiation Medium is used to obtain morphologically relevant airway organoids. Airway organoids provide a functional in vitro organotypic culture system for studying the airway epithelium. When fully differentiated, the organoids exhibit a centralized lumen surrounded by a polarized airway epithelial cell layer, comprised of differentiated cell types, such as ciliated cells and goblet cells. Applications of airway organoid cultures include modeling lung diseases, drug screening for efficacy and toxicity, and studying the functions of the airway epithelium.

Browse our Frequently Asked Questions (FAQs) about culturing airway organoids culture using PneumaCult™.

Should you intend to use this product for commercial purposes, please contact HUB at www.huborganoids.nl for a commercial use license or for clarifications in relation to HUB licensing.
Subtype
Specialized Media
Cell Type
Airway Cells
Species
Human
Application
Cell Culture, Differentiation, Expansion, Organoid Culture
Brand
PneumaCult
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids, Respiratory Research
Formulation Category
Serum-Free

Data Figures

Schematic of human airway organoid culture workflow using PneumaCult™ Airway Organoid Kit

Figure 1. Overview of the PneumaCult™ Culture System for Human Airway Organoid Generation

In the early two-dimensional expansion phase of the human airway organoid culture procedure, HBECs are expanded using PneumaCult™-Ex Plus Medium. The HBECs are then embedded into a Matrigel® dome and expanded for 4 - 7 days using PneumaCult™ Airway Organoid Seeding Medium. Following the expansion, the HBECs are differentiated using PneumaCult™ Airway Organoid Differentiation Medium for an additional 21+ days.

Fully differentiated human airway organoids generated using PneumaCult™ Airway Organoid Kit display physiologically relevant morphology.

Figure 2. Fully Differentiated Human Airway Organoids Generated Using PneumaCult™ Airway Organoid Kit

(A) Bright-field image of airway organoids growing in PneumaCult™ Airway Organoid Seeding Medium at day 7 exhibit basal cell spheroid morphology. (B) Bright-field image of airway organoids differentiated in PneumaCult™ Airway Organoid Differentiation Medium at day 21 exhibit hollow lumens. (C) Airway organoid stained for ZO-1 (junction protein marker, red), MUC5AC (goblet cell marker, purple), AC-Tubulin (ciliated cell marker, green), and DAPI (nuclei, blue).

Comparison of Forskolin-treated airway organoids derived from healthy donors and CF donors.

Figure 3. Forskolin-Induced Swelling of Airway Organoids

(A) Forskolin-treated organoids derived from healthy donors increased in size compared to the DMSO control, indicating functional CFTR protein expression. (B) Forskolin-induced swelling is lost in organoids derived from CF donors, but re-established in VX-809-treated airway organoids. Error bars represent ± 95% confidence interval for the mean (n=3). Bright-field images of airway organoids taken during the Forskolin swelling assay at (C) 0 hours and (D) 6 hours show organoid swelling after treatment.

Ciliated cell percentage in fully differentiated airway organoids derived from healthy and cystic fibrosis (CF) donors using PneumaCult™ Airway Organoid Kit at different passages.

Figure 4. Fully Differentiated Airway Organoids Retain Morphological Characteristics at Different Passages

The ciliated cell percentage in organoids grown from (A) healthy and (B) CF donors using PneumaCult™ Airway Organoid Kit increased from P3 to P5. The total and ciliated cells were counted using a hemocytometer. Error bars represent ± 95% confidence interval for the mean (n=3).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
PneumaCult™ Airway Organoid Kit
Catalog #
05060
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
PneumaCult™ Airway Organoid Kit
Catalog #
05060
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
PneumaCult™ Airway Organoid Kit
Catalog #
05060
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
PneumaCult™ Airway Organoid Kit
Catalog #
05060
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids
View All

Resources and Publications

Educational Materials (22)

Pulmonary Research
Brochure
Pulmonary Research
Organoids
Brochure
Organoids
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
Protocol
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
Performing Immunocytochemical Staining of Epithelial Organoids
Protocol
Performing Immunocytochemical Staining of Epithelial Organoids
Dissociation of Mouse Lung Tissue into a Single-Cell Suspension
Protocol
Dissociation of Mouse Lung Tissue into a Single-Cell Suspension
Forskolin-Induced Swelling of Airway Organoids Derived from Healthy and Cystic Fibrosis Donors
Protocol
Forskolin-Induced Swelling of Airway Organoids Derived from Healthy and Cystic Fibrosis Donors
Dynamic Modeling in Organoids
Wallchart
Dynamic Modeling in Organoids
Cellular Organization and Biology of the Respiratory System
Wallchart
Cellular Organization and Biology of the Respiratory System
What Are the Impacts of New Legislative Actions on Drug Development?
3:07
Video
What Are the Impacts of New Legislative Actions on Drug Development?
Beating Cilia of Airway Organoids Grown in PneumaCult™
0:30
Video
Beating Cilia of Airway Organoids Grown in PneumaCult™
Using Stem Cell-Based Models in Drug Discovery
46:49
Webinar
Using Stem Cell-Based Models in Drug Discovery
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
1:10:09
Webinar
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
Human Airway Organoids to Study Host-Pathogen Interactions
35:24
Webinar
Human Airway Organoids to Study Host-Pathogen Interactions
Studying Cystic Fibrosis Using Primary Human Nasal Epithelial Cells
51:30
Webinar
Studying Cystic Fibrosis Using Primary Human Nasal Epithelial Cells
Human Lung Development and Disease: Lessons from Organoids
26:16
Webinar
Human Lung Development and Disease: Lessons from Organoids
In Vitro Human Airway Modeling Using Primary Airway Epithelial Cells
18:41
Webinar
In Vitro Human Airway Modeling Using Primary Airway Epithelial Cells
Optimization of Normal Human Bronchial Epithelial Cell 3D Cultures for In Vitro Lung Model Studies
12:13
Webinar
Optimization of Normal Human Bronchial Epithelial Cell 3D Cultures for In Vitro Lung Model Studies
Organoid-Based Assays for Disease Modeling
24:55
Webinar
Organoid-Based Assays for Disease Modeling
Reliable In Vitro Culture Solutions for Your Alveolar Research
18:52
Webinar
Reliable In Vitro Culture Solutions for Your Alveolar Research
Studying Respiratory Viruses with ALI Cultures
6:56
Webinar
Studying Respiratory Viruses with ALI Cultures
Elucidating Parechovirus A Pathogenesis Using Organoid Models
30:09
Webinar
Elucidating Parechovirus A Pathogenesis Using Organoid Models
Dr. Bas Trietsch
Interview
Dr. Bas Trietsch

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Interested in trying STEMCELL's organoid products for respiratory research? Fill out this form to request information about introductory offers.

Legal Statement:

This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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