Optimization of Normal Human Bronchial Epithelial Cell 3D Cultures for In Vitro Lung Model Studies
Robust, physiologically relevant in vitro lung model systems are required to properly investigate respiratory physiology and pathology. However, obtaining truly differentiated air-liquid-interface (ALI) cultures with airway epithelial cells can be challenging, due to the limited expansion and differentiation potential associated with primary cells. In this virtual paper presentation, Dr. Rachael Rayner evaluates the effects of different expansion media on the ability to expand primary normal human bronchial epithelial cells while maintaining their 3D culture characteristics. Her research highlights the need for more critical assessments of expansion and differentiation culture conditions to obtain optimal morphology and functional readouts.
Publish Date:
August 23, 2019
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Serum- and BPE-free medium for human small airway epithelial cells cultured at the air-liquid interface -
PneumaCult™ Airway Organoid Kit
Serum- and BPE-free medium for efficient establishment and differentiation of airway organoids
