D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

D-PBS (Without Ca++ and Mg++)

Dulbecco’s phosphate-buffered saline without calcium and magnesium

D-PBS (Without Ca++ and Mg++)
500 mL
37 USD
Catalog # 37350

Dulbecco’s phosphate-buffered saline without calcium and magnesium

D-PBS, 10X Concentrate (Without Ca++ and Mg++)
500 mL
56 USD
Catalog # 37354

Dulbecco’s phosphate-buffered saline, 10X concentrate, without calcium and magnesium

Overview

Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating, or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions.
Species
Human, Mouse, Rat, Non-Human Primate, Other

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
37350
Lot #
All
Language
English
Catalog #
37354
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
37350
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
37354
Lot #
All
Language
English

Data and Publications

Publications (1)

In situ label-free quantification of human pluripotent stem cells with electrochemical potential Yea C-H et al. Biomaterials 2016 JAN

Abstract

Conventional methods for quantification of undifferentiated pluripotent stem cells such as fluorescence-activated cell sorting and real-time PCR analysis have technical limitations in terms of their sensitivity and recyclability. Herein, we designed a real-time in situ label-free monitoring system on the basis of a specific electrochemical signature of human pluripotent stem cells in vitro. The intensity of the signal of hPSCs highly corresponded to the cell number and remained consistent in a mixed population with differentiated cells. The electrical charge used for monitoring did not markedly affect the proliferation rate or molecular characteristics of differentiated human aortic smooth muscle cells. After YM155 treatment to ablate undifferentiated hPSCs, their specific signal was significantly reduced. This suggests that detection of the specific electrochemical signature of hPSCs would be a valid approach to monitor potential contamination of undifferentiated hPSCs, which can assess the risk of teratoma formation efficiently and economically.

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