Cord Blood Banking
Numerous clinical studies have shown that the total number of CFUs in a CB unit is the one parameter that best correlates with the time to neutrophil and platelet engraftment, and overall survival following unrelated allogeneic CB transplantation.1-6
Products
Cell Culture Media and Supplements (4 9)
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MethoCult™ H4434 Classic
Methylcellulose-based medium with recombinant cytokines for human cells -
MethoCult™ Express
Methylcellulose-based medium with recombinant cytokines for rapid hematopoietic colony assays of human cells -
MethoCult™ H4534 Classic Without EPO
Methylcellulose-based medium with recombinant cytokines (without erythropoietin [EPO]) for human cells -
Starter Kit for MethoCult™ H4534 Classic Without EPO
Complete kit for hematopoietic CFU assays
Instruments and Software (4 1)
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STEMvision™
Automated and standardized colony counting for the hematopoietic colony-forming unit (CFU) assay
Antibodies (4 1)
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Anti-Human CD34 Antibody, Clone 8G12
Mouse monoclonal IgG1 antibody against human CD34
Cell Dyes and Detection Assay Kits (4 5)
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ALDHbr Assay Kit
For the detection of CD34+ and ALDHbr cells in human cord blood -
ALDEFLUOR™ Kit
For the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH -
7-AAD (7-Aminoactinomycin D)
Cell viability dye (DNA-labeling dye) -
Trypan Blue
Reagent for counting viable mammalian cells
Cultureware and General Supplies (4 9)
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245 mm Square Dish, Tissue Culture-Treated
Sterile clear polystyrene tissue culture-treated dish with lid -
STEMgrid™-6
Detachable grid for manual counting of hematopoietic colonies in SmartDish™ 6-well plates using an inverted microscope -
Corning® 245 mm Square Dish, Non-Treated
Square dish without handles -
SmartDish™
Meniscus-free cultureware for more accurate counting of hematopoietic colonies in CFU assays
Buffers and Solutions (4 5)
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ALDEFLUOR™ Assay Buffer
Assay buffer for labeling with the ALDEFLUOR™ kit -
Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum
Cell culture buffer -
HBSS with 10 mM HEPES, Without Phenol Red
Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red -
HBSS, Modified (Without Ca++ and Mg++)
Hanks' Balanced Salt Solution (HBSS) without calcium and magnesium
Cell Storage Media (4 3)
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BloodStor® 55-5
Optimized biopreservation reagent for hematopoietic cells and tissues -
BloodStor® 100
Biopreservation reagent for cells and tissues -
HypoThermosol® FRS
Animal component-free, defined hypothermic (2 - 8°C) preservation medium for a range of cell and tissue types
Standardization Tools (4 4)
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Atlas of Hematopoietic Colonies From Cord Blood
Atlas of cord blood-derived colonies in methylcellulose -
Frozen Cord Blood Proficiency Testing Program
For the hematopoietic colony forming unit (CFU) assay -
Human Cord Blood Quality Control Kit
Quality control kit for hematopoietic colony assays -
Fresh Cord Blood Proficiency Testing Program
For the hematopoietic colony forming unit (CFU) assay