Cord Blood Banking

Numerous clinical studies have shown that the total number of CFUs in a CB unit is the one parameter that best correlates with the time to neutrophil and platelet engraftment, and overall survival following unrelated allogeneic CB transplantation.1-6 See MoreSeveral regulatory bodies (e.g. FDA, AABB, NetCord-FACT) recommend or require that the number of CFUs in a CB unit be measured before cryopreservation and/or after thawing, prior to release of the unit to a transplant facility. Tools for standardization of the CFU assay include semi-solid methylcellulose-based medium, specialized cultureware and an instrument for automated counting of CFU assays. MethoCult™ methylcellulose-based medium is formulated to consistently stimulate the growth and differentiation of progenitor cells, while restricting the migration of progeny so that colonies are formed. SmartDish™ meniscus-free cultureware has been developed to allow for more accurate colony counting for manual or automated counting of CFU assays. By combining SmartDish™ with our instrument for automated colony counting and scoring, cord blood banks can ensure that their CFU assay results are more accurate and reproducible compared to those counted manually.

A new flow cytometry assay, the ALDHbr assay, was recently validated for its utility in measuring the frequency of viable, metabolically active HSPCs in CB.7 This assay combines the measurement of a cell’s aldehyde dehydrogenase (ALDH) activity with staining for cell surface marker expression and has been shown to correlate with CFU assay results.


  1. Migliaccio A et al. (2000) Blood 96: 2717-2722
  2. Iori A et al. (2004) Bone Marrow Transpl 33: 1097-1105
  3. Yoo K et al. (2007) Bone Marrow Transpl 39: 515 521
  4. Prasad V et al. (2008) Blood 112: 2979-2989
  5. Page K et al. (2011) Biol Blood Marrow Tr 17: 1362-1374
  6. Pamphilon D et al. (2013) Cytotherapy, 15: 255-262
  7. Shoulars K et al. (2016) Blood, 127: 2346-2354
See Less


Cell Culture Media and Supplements 4 (9)

Instruments and Software 4 (1)

  • STEMvision™ Instrument


    Automated and standardized colony counting for the hematopoietic colony-forming unit (CFU) assay

Antibodies 4 (1)

Cell Dyes and Detection Assay Kits 4 (6)

Cultureware and General Supplies 4 (7)

Buffers and Solutions 4 (5)

Cell Storage Media 4 (3)

Standardization Tools 4 (4)

Uncategorized 4 (1)

  • STEMgrid™-6


    Detachable grid for manual counting of hematopoietic colonies in SmartDish™ 6-well plates using an inverted microscope

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.