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Cord Blood Banking

Numerous clinical studies have shown that the total number of CFUs in a CB unit is the one parameter that best correlates with the time to neutrophil and platelet engraftment, and overall survival following unrelated allogeneic CB transplantation.1-6 See MoreSeveral regulatory bodies (e.g. FDA, AABB, NetCord-FACT) recommend or require that the number of CFUs in a CB unit be measured before cryopreservation and/or after thawing, prior to release of the unit to a transplant facility. Tools for standardization of the CFU assay include semi-solid methylcellulose-based medium, specialized cultureware and an instrument for automated counting of CFU assays. MethoCult™ methylcellulose-based medium is formulated to consistently stimulate the growth and differentiation of progenitor cells, while restricting the migration of progeny so that colonies are formed. SmartDish™ meniscus-free cultureware has been developed to allow for more accurate colony counting for manual or automated counting of CFU assays. By combining SmartDish™ with our instrument for automated colony counting and scoring, cord blood banks can ensure that their CFU assay results are more accurate and reproducible compared to those counted manually.

A new flow cytometry assay, the ALDHbr assay, was recently validated for its utility in measuring the frequency of viable, metabolically active HSPCs in CB.7 This assay combines the measurement of a cell’s aldehyde dehydrogenase (ALDH) activity with staining for cell surface marker expression and has been shown to correlate with CFU assay results.

References

  1. Migliaccio A et al. (2000) Blood 96: 2717-2722
  2. Iori A et al. (2004) Bone Marrow Transpl 33: 1097-1105
  3. Yoo K et al. (2007) Bone Marrow Transpl 39: 515 521
  4. Prasad V et al. (2008) Blood 112: 2979-2989
  5. Page K et al. (2011) Biol Blood Marrow Tr 17: 1362-1374
  6. Pamphilon D et al. (2013) Cytotherapy, 15: 255-262
  7. Shoulars K et al. (2016) Blood, 127: 2346-2354
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Cell Culture Media and Supplements (49)

Instruments and Software (41)

  • STEMvision™ Human Cord Blood 7-Day CFU Analysis Package|22001

    STEMvision™

    Automated and standardized colony counting for the hematopoietic colony-forming unit (CFU) assay

Antibodies (41)

Cell Dyes and Detection Assay Kits (45)

Cultureware and General Supplies (49)

Buffers and Solutions (45)

Cell Storage Media (43)

  • BloodStor® 55-5|07937

    BloodStor® 55-5

    Optimized biopreservation reagent for hematopoietic cells and tissues
  • BloodStor® 100|07939

    BloodStor® 100

    Biopreservation reagent for cells and tissues
  • HypoThermosol® FRS|07935

    HypoThermosol® FRS

    Animal component-free, defined hypothermic (2 - 8°C) preservation medium for a range of cell and tissue types

Standardization Tools (44)