Meniscus-free cultureware for more accurate counting of hematopoietic colonies in CFU assays
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SmartDish™ (Catalog #27370) is the second generation of the original SmartDish™ plate (Catalog #27301). These new plates use an improved coating, which streamlines the manufacturing process while consistently improving the visibility of hematopoietic colonies in culture through elimination of the mensicus. Current SmartDish™ customers can contact their local STEMCELL representative for more information.
SmartDish™ has been designed for both manual counting with STEMgrid™-6 as well as automated colony counting with a STEMvision™ instrument.
Browse our Frequently Asked Questions (FAQs) on performing the CFU assay.
Figure 1. Manual and Automated Counting of CFU Assays in Standard and SmartDish™ Culture Plates
Shown are representative 35 mm wells from (A) standard and (B) SmartDish™ culture plates with STEMgrid-6 attached to facilitate colony counting on an inverted microscope, or (C) standard and (D) SmartDish™ plates imaged using STEMvision™. Optical distortion caused by the meniscus obscures colonies at the edge of cultures grown in standard plates (A, C) and makes them more difficult to count (see circled colony in A). Colonies are more easily seen and counted at the edge of the SmartDish™ wells which have been treated to eliminate meniscus formation (B, D).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.