SmartDish™

Meniscus-free cultureware for more accurate counting of hematopoietic colonies in CFU assays

SmartDish™

Meniscus-free cultureware for more accurate counting of hematopoietic colonies in CFU assays

From: 81 USD
Catalog #
27370_C
Meniscus-free cultureware for more accurate counting of hematopoietic colonies in CFU assays

Overview

SmartDish™ plates enable accurate and reproducible hematopoietic colony counting by preventing the formation of a meniscus. This allows for an even distribution of culture medium, resulting in a more uniform distribution of colonies throughout the entire 35 mm well. The absence of the meniscus also reduces optical distortion so that colonies located at the edge can be more easily counted.

SmartDish™ (Catalog #27370) is the second generation of the original SmartDish™ plate (Catalog #27301). These new plates use an improved coating, which streamlines the manufacturing process while consistently improving the visibility of hematopoietic colonies in culture through elimination of the mensicus. Current SmartDish™ customers can contact their local STEMCELL representative for more information.

SmartDish™ has been designed for both manual counting with STEMgrid™-6 as well as automated colony counting with a STEMvision™ instrument.

Browse our Frequently Asked Questions (FAQs) on performing the CFU assay.
Subtype
Dishes and Plates
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Cell Culture, Colony Assay
Brand
SmartDish

Data Figures

Manual Counting of Hematopoietic CFU Assays

Manual Counting of Hematopoietic CFU Assays

Automated Counting of Hematopoietic CFU Assays

Automated Counting of Hematopoietic CFU Assays

Figure 1. Manual and Automated Counting of CFU Assays in Standard and SmartDish™ Culture Plates

Shown are representative 35 mm wells from (A) standard and (B) SmartDish™ culture plates with STEMgrid-6 attached to facilitate colony counting on an inverted microscope, or (C) standard and (D) SmartDish™ plates imaged using STEMvision™. Optical distortion caused by the meniscus obscures colonies at the edge of cultures grown in standard plates (A, C) and makes them more difficult to count (see circled colony in A). Colonies are more easily seen and counted at the edge of the SmartDish™ wells which have been treated to eliminate meniscus formation (B, D).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
27370
Lot #
All
Language
English
Catalog #
27371
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Contact STEMCELL Technologies

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