Human Pluripotent Stem Cell Research

Induced pluripotent stem (iPS) cells are a type of pluripotent stem cell (PSC) that can be obtained by reprogramming somatic cells through artificial expression of key transcription factors under specific culture conditions.1-2 See More Variables that affect reprogramming include the delivery method, culture conditions and starting cell type. The choice of starting cell type is influenced by factors such as availability of donor tissue, invasiveness of sample collection procedures, genomic integrity, epigenetic memory and reprogramming efficiency. For efficient reprogramming, use our non-viral and non-integrating vector system, TeSR-based media, and small molecules. For blood-derived cells, we offer integrated kits to enrich, isolate, expand and reprogram erythroid progenitor or CD34+ progenitor cells. For help with your hPSC workflow decision making, use our infographics to find the right reagents for you.

References

  1. Takahashi K, et al. Cell 131(5): 861–72, 2007
  2. Yu J, et al. Science 318(5858): 1917–20, 2007
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Products

Cell Culture Media and Supplements (48)

  • ReproRNA™-OKSGM Kit

    ReproRNA™-OKSGM

    A non-integrating, self-replicating RNA reprogramming vector for generating iPS cells
  • TeSR™-E7™ Medium for Reprogramming

    TeSR™-E7™

    Feeder-free, xeno-free reprogramming medium for human iPS cell induction
  • ReproTeSR™

    ReproTeSR™

    Reprogramming medium for human iPS cell induction
  • Erythroid Progenitor Reprogramming Kit

    Erythroid Progenitor Reprogramming Kit

    Integrated workflow containing tools to isolate and expand erythroid progenitor cells from peripheral blood and their subsequent reprogramming to iPS cells

Matrices and Substrates (43)

  • CellAdhere™ Laminin-521

    CellAdhere™ Laminin-521

    Matrix for maintenance of human ES and iPS cells in combination with mTeSR™1, TeSR™-E8™, or TeSR™2
  • Vitronectin XF™

    Vitronectin XF™

    Defined, xeno-free cell attachment factor that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions
  • 0.1% Gelatin in Water

    0.1% Gelatin in Water

    Coating for cultureware

Small Molecules (422)

  • CHIR99021

    CHIR99021

    WNT pathway activator; Inhibits GSK3
  • PD0325901

    PD0325901

    MEK/ERK pathway inhibitor; Inhibits MEK
  • SB431542

    SB431542

    Activin/BMP/TGFβ pathway inhibitor; Inhibits ALK4, ALK5, and ALK7
  • Valproic Acid (Sodium Salt)

    Valproic Acid

    Epigenetic modifier; Inhibits histone deacetylase (HDAC)1

Primary and Cultured Cells (43)

Tissue and Cell Culture Dissociation Reagents (42)

Cell Storage Media (45)

  • mFreSR™

    mFreSR™

    Defined, serum-free cryopreservation medium for human ES and iPS cells
  • CryoStor® CS2

    CryoStor® CS2

    Animal component-free, defined cryopreservation medium with 2% DMSO
  • CryoStor® CS5

    CryoStor® CS5

    Animal component-free, defined cryopreservation medium with 5% DMSO
  • CryoStor® CS10|07930

    CryoStor® CS10

    Animal component-free, defined cryopreservation medium with 10% DMSO

Antibodies (47)

Cytokines (43)

Cultureware and General Supplies (41)

Cell Isolation Products (45)

Buffers and Solutions (41)