EasySep™ Human CD34 Positive Selection Kit II

Immunomagnetic positive selection kit

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From: 699 USD

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Immunomagnetic positive selection kit
From: 699 USD

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Overview

The EasySep™ Human CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh or previously frozen bone marrow or mobilized peripheral blood mononuclear cells, or from previously frozen cord blood mononuclear cells, or from hESC/hiPSC-derived hematopoietic cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.

This product can be used in place of the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components:
  • EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856)
    • EasySep™ Human CD34 Positive Selection Cocktail, 1 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 x 1 mL
  • RoboSep™ Human CD34 Positive Selection Kit II with Filter Tips (Catalog #17856RF)
    • EasySep™ Human CD34 Positive Selection Cocktail, 1 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
 
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Hematopoietic Stem and Progenitor Cells; Pluripotent Stem Cells
Species:
Human
Sample Source:
Bone Marrow; Cord Blood; Other; PBMC; Whole Blood; Pluripotent Stem Cells
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology; Stem Cell Biology; Chimerism

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with cord blood, mobilized peripheral blood or bone marrow MNCs, or ES and iPS cell cultures, the CD34+ cell content of the isolated fraction is typically 93.5 ± 1.1% (mean ± SD using the purple EasySep™ Magnet). In the above example using frozen cord blood, the purities of the start and final isolated fractions are 2.2% and 94.7%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.