How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)

This step-by-step technical guide demonstrates how to isolate peripheral blood mononuclear cells (PBMCs) from whole blood using density gradient centrifugation (e.g. Ficoll-Paque™ or Lymphoprep™). See how to layer blood over the density medium and learn two different ways of harvesting cells from the blood plasma:density medium interface. Learn how SepMate™ tubes can speed up and simplify your density gradient centrifugation. After isolating PBMCs, you can isolate highly purified cell subsets using the EasySep™ magnetic cell separation.

See a written version of this protocol >

Publish Date: March 13, 2012 Views: 180103

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