How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
This step-by-step technical guide demonstrates how to isolate peripheral blood mononuclear cells (PBMCs) from whole blood using density gradient centrifugation (e.g. Ficoll-Paque™ or Lymphoprep™).
See how to layer blood over the density medium and learn two different ways of harvesting cells from the blood plasma:density medium interface.
Learn how SepMate™ tubes can speed up and simplify your density gradient centrifugation.
After isolating PBMCs, you can isolate highly purified cell subsets using the EasySep™ magnetic cell separation.
Publish Date:
March 13, 2012
Related Multimedia
-
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
Publish Date: March 23, 2018 -
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
Publish Date: May 07, 2012
Explore Our Products
-
EasySep™ Mouse APC Positive Selection Kit II
Immunomagnetic positive selection cell isolation kit -
EasySep™ Human CD56 Positive Selection Kit II
Immunomagnetic positive selection cell isolation kit -
EasySep™ Human EpCAM Positive Selection Kit II
Immunomagnetic positive selection kit -
EasySep™ Human CD4+ T Cell Enrichment Kit
Immunomagnetic negative selection kit
