When added to serum-free medium, the CD34+ Expansion Supplement typically promotes ~40-fold expansion of total nucleated cells in 7-day liquid cultures of CD34+ human CB cells. After one week, ~40% of the cultured cells express CD34, indicative of 10-fold expansion of input CD34+ CB cells. This supplement stimulates greater CD34+ cell expansion when compared to StemSpan™ CC100 and CC110. See data tab for more details.
• Optimized for use with StemSpan™ media. When combined with StemSpan™ SFEM II in particular, supports at least 50% higher expansion of CD34+ human CB cells when compared to other serum-free media on the market.
• Supplied as a 10X concentrate. After thawing and mixing, the tube contents can be added directly to any hematopoietic cell expansion medium of choice.
• Recombinant human stem cell factor (SCF)
• Recombinant human interleukin 3 (IL-3)
• Recombinant human interleukin 6 (IL-6)
• Recombinant human thrombopoietin (TPO)
• Other additives
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Data and Publications
Table 1. HSC Expansion Culture with CD34+ Human Cord Blood Cells Cultured in StemSpan™ SFEM Containing CD34+ Expansion Supplement
Shown are the percent CD34+ cells, fold expansion of total nucleated cells (TNC) and CD34+ cells, and numbers of colony-forming units (CFU) produced per input CD34+ cell after 7 days of hsc expansion culture of enriched CD34+ cells from six independent cord blood (CB) samples.
*95% confidence limits, the range within which 95% of the results will typically fall.
ND: not done
Figure 1. Comparison of CD34+ Cell Expansion in Different StemSpan™ Media Containing CD34+ Expansion Supplement
Average expansion of (A) total nucleated cells (TNC), (B) CD34+ cells and (C) colony-forming units (CFU), normalized relative to the values obtained in StemSpan™ SFEM (grey bars) after culturing purified hematopoietic CD34+ cord blood cells (n=6) for 7 days in StemSpan™ SFEM, SFEM II (gold bars) or ACF (orange bars) media containing CD34+ Expansion Supplement. Vertical lines indicate 95% confidence limits, the range within which 95% of results will typically fall. Cell yields in StemSpan™ SFEM II were on average ~60% higher than in StemSpan™ SFEM and StemSpan™ ACF.
*p<0.001, #p<0.05 (paired t-test, n=6 in A and B, n=4 in C).