Anti-Human CD34 Antibody, Clone 581

Mouse monoclonal IgG1 antibody against human CD34

Anti-Human CD34 Antibody, Clone 581

Mouse monoclonal IgG1 antibody against human CD34

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Mouse monoclonal IgG1 antibody against human CD34
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Overview

The 581 antibody reacts with human CD34, an ~105 - 120 kDa type 1 transmembrane glycoprotein expressed on the surface of most human hematopoietic stem and progenitor cells (HSPCs) as well as on mesenchymal stem cells, embryonic fibroblasts, endothelial cells, neurons, and some tumor cell lines. CD34 is expressed only transiently during hematopoiesis, so the frequency of CD34+ cells is low in bone marrow or cord blood (~1 - 5%) and very low (~0.1 - 0.5%) in peripheral blood. CD34 is a marker used to identify and isolate HSPCs capable of cell engraftment. CD34 is thought to mediate attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells during early hematopoiesis, and to be involved in lymphocyte recruitment through binding to the ligands L- and E-selectin. Distinct epitope groups have been assigned to CD34 based on their sensitivity to enzymatic cleavage, with the 581 antibody recognizing a class III epitope (resistant to neuraminidase and O-glycoprotease).

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ Human CD34 Positive Selection Kit (Catalog #18056) and for labeling human mesenchymal cells grown in MesenCult™ Proliferation Kit (Human; Catalog #05411).
Subtype
Primary Antibodies
Target Antigen
CD34
Alternative Names
Gp105-120, My10
Reactive Species
Human
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Application
Cell Isolation, CyTOF, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Area of Interest
Stem Cell Biology
Clone
581
Gene ID
947
Isotype
IgG1, kappa

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD34 Antibody, Clone 581, Alexa Fluor® 488 and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of PBMCs labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (Catalog #60070AD)and Anti-Human CD45 Antibody, Clone HI30, APC. (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD34 Positive Selection Kit and labeled with Anti-Human CD34 Antibody, Clone 581, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 is shown (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD34 Antibody, Clone 581, APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE). (B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG1, kappa APC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, PE. (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD34 Positive Selection Kit and labeled with Anti-Human CD34 Antibody, Clone 581, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD34 Antibody, Clone 581, Biotin followed by streptavidin (SAV) APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE).
(B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC and Anti-Human CD45 Antibody, Clone HI30, PE.

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD34 Antibody, Clone 581, FITC and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG1, kappa FITC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, APC. (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD34 Positive Selection Kit and labeled with Anti-Human CD34 Antibody, Clone 581, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD34 Antibody, Clone 581, PE and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of PBMCs labeled with a mouse IgG1, kappa PE isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, APC. (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD34 Positive Selection Kit and labeled with Anti-Human CD34 Antibody, Clone 581, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).

Data for Unconjugated

Figure 6. Data for Unconjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD34 Antibody, Clone 581, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC and Anti-Human CD45 Antibody, Clone HI30, APC. (C) Flow cytometry analysis of human KG1a myeloid cells labeled with Anti-Human CD34 Antibody, Clone 581, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (filled histogram), or a mouse IgG1, kappa isotype control antibody followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60013FI.1, 60013FI
Lot #
All
Language
English
Catalog #
60013BT.1, 60013BT
Lot #
All
Language
English
Catalog #
60013AZ.1, 60013AZ
Lot #
All
Language
English
Catalog #
60013PE.1, 60013PE
Lot #
All
Language
English
Catalog #
60013AD.1, 60013AD
Lot #
All
Language
English
Catalog #
60013
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60013FI.1, 60013FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60013BT.1, 60013BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60013AZ.1, 60013AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60013PE.1, 60013PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60013AD.1, 60013AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60013
Lot #
All
Language
English