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Data and Publications
(A) The biological activity of Human Recombinant EGFR was tested by its ability to promote the proliferation of BALB/c 3T3 cells in the presence of 25 pg/mL EGF. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.81 µg/mL. (B) 2 μg of Human Recombinant EGFR was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant EGFR has a predicted molecular mass of 69.4 kDa.