Anti-Human SSEA-4 Antibody, Clone MC-813-70
Mouse monoclonal IgG3 antibody against human, mouse, rat SSEA-4
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Overview
This antibody clone has been verified for labeling human ES and iPS cells grown in mTeSR™1 (Catalog #85850), and TeSR™2 (Catalog #05860), and has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human ES/iPS Cell TRA-1-60 Positive Selection Kit (Catalog #18166) and EasySep™ hESC/hiPSC SSEA-4 Positive Selection Kit (Catalog #18165; partial blocking may be observed).
Data Figures

Figure 1. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-4 Antibody, Clone MC-813-70, Alexa Fluor® 488. Labeling of human ES cells with a mouse IgG3, kappa Alexa Fluor® 488 isotype control antibody is shown (solid line histogram).
(B) Human ES cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70, Alexa Fluor® 488. Inset shows cells labeled with a mouse IgG3, kappa, Alexa Fluor® 488 isotype control antibody.
(C) Flow cytometry analysis of human iPS cells labeled with Anti-Human SSEA-4 Antibody, Clone MC-813-70, Alexa Fluor® 488 (filled histogram) or a mouse IgG3, kappa Alexa Fluor® 488 isotype control antibody (open histogram).
(D) Human iPS cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70, Alexa Fluor® 488. Inset shows cells labeled with a mouse IgG3, kappa Alexa Fluor® 488 isotype control antibody.

Figure 2. Data for Biotin-Conjugated
Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-4 Antibody, Clone MC-813-70, Biotin followed by streptavidin (SAV) APC (filled histogram). Labeling of human ES cells with a mouse IgG3, kappa biotin isotype control antibody followed by SAV APC is shown (solid line histogram).

Figure 3. Data for FITC-Conjugated
Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti- Human SSEA-4 Antibody, Clone MC-813-70, FITC (filled histogram). Labeling of human ES cells with a mouse IgG3, kappa FITC isotype control antibody is shown (solid line histogram).

Figure 4. Data for PE-Conjugated
(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-4 Antibody, Clone MC-813-70, PE. Labeling of human ES cells with a mouse IgG3, kappa PE isotype control antibody is shown (solid line histogram).
(B) Human ES cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70, PE. Inset shows cells labeled with a mouse IgG3, kappa, PE isotype control antibody.
(C) Flow cytometry analysis of human iPS cells labeled with Anti-Human SSEA-4 Antibody, Clone MC-813-70, PE (filled histogram) or a mouse IgG3, kappa PE isotype control antibody (open histogram).

Figure 5. Data for Unconjugated
(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-4 Antibody, Clone MC-813-70 followed by goat anti-mouse IgG, FITC. Labeling of human ES cells with a mouse IgG3, kappa isotype control antibody followed by goat anti-mouse IgG FITC is shown (solid line histogram).
(B) Human ES cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG3, kappa isotype control antibody.
(C) Flow cytometry analysis of human iPS cells labeled with Anti-Human SSEA-4 Antibody, Clone MC-813-70 followed by goat anti-mouse IgG FITC (filled histogram) or a mouse IgG3, kappa isotype control antibody followed by goat anti-mouse IgG FITC (open histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
Publications (6)
Abstract
Abstract
Abstract
Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.