Mouse IgG3, kappa Isotype Control Antibody, Clone MG3- 35

Mouse monoclonal IgG3, kappa isotype control antibody

Mouse IgG3, kappa Isotype Control Antibody, Clone MG3- 35

Mouse monoclonal IgG3, kappa isotype control antibody

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Mouse monoclonal IgG3, kappa isotype control antibody
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Overview

The MG3-35 antibody (IgG3, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Subtype
Isotype Controls
Target Antigen
IgG3 Isotype Control
Alternative Names
Not applicable
Conjugation
Alexa Fluor 488, Biotin, PE, Unconjugated
Host Species
Mouse
Cell Type
Other
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
MG3-35
Gene ID
Not applicable
Isotype
IgG3, kappa

Data Figures

Data for unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human iPS cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, followed by a goat anti-mouse IgG antibody, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, Catalog #60062) followed by a goat anti-mouse IgG antibody, FITC. (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70 (Catalog #60062), followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human iPS cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, Alexa Fluor® 488, Catalog #60062AD). B) Human ES cells were cultured in mTeSR™1 on Corning Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70, Alexa Fluor® 488. Inset shows cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Alexa Fluor® 488.

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of human ES cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, Biotin, Catalog #60062BT) followed by SAV APC.

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of human ES cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, FITC, Catalog #60062FI).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

(A) Flow cytometry analysis of human iPS cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG3, kappa positive control antibody (Anti-Human SSEA-4 Antibody, Clone MC-813-70, PE, Catalog #60062PE). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-4 Antibody, Clone MC-813-70, PE (Catalog #60062PE). Inset shows cells labeled with Mouse IgG3, kappa Isotype Control Antibody, Clone MG3-35.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60073BT.1, 60073BT
Lot #
All
Language
English
Catalog #
60073, 60073.1
Lot #
All
Language
English
Catalog #
60073PE, 60073PE.1
Lot #
All
Language
English
Catalog #
60073AD, 60073AD.1
Lot #
All
Language
English
Catalog #
60073FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60073BT.1, 60073BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60073, 60073.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60073PE, 60073PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60073AD, 60073AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60073FI
Lot #
All
Language
English

Resources and Publications