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Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173

Mouse monoclonal IgG2a, kappa isotype control antibody

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Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173

Mouse monoclonal IgG2a, kappa isotype control antibody

From: 52 USD
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Mouse monoclonal IgG2a, kappa isotype control antibody
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Overview

The MOPC-173 antibody (IgG2a, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Subtype
Isotype Controls
Target Antigen
IgG2a Isotype Control
Alternative Names
Not applicable
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Other
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
MOPC-173
Gene ID
Not applicable
Isotype
IgG2a, kappa

Data Figures

Flow Cytometry Analysis of Human PBMCs

Figure 1. Flow Cytometry Analysis of Human PBMCs

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, followed by a rat anti-mouse IgG2a antibody, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, Catalog #60097) followed by a rat anti-mouse IgG2a antibody, PE. (B) E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit (Catalog #05712) on poly-lysine-coated glass coverslips, then fixed and labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, followed by donkey anti-mouse IgG, Alexa Fluor® 488 and DAPI counterstaining (inset), or with a positive control antibody of the same isotype, Anti-Human Beta-Tubulin III Antibody, Clone AA10 (Catalog #60100), followed by donkey anti-mouse IgG, Alexa Fluor® 488 (green) and DAPI counterstaining (blue).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, Alexa Fluor® 488, Catalog #60097AD). (B) E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit (Catalog #05712) on poly-lysine-coated glass coverslips, then fixed and labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Inset) or with a positive control antibody of the same isotype, Anti-Beta-Tubulin III Antibody, Clone AA10, Alexa Fluor® 488 (Catalog #60100AD, green). DAPI counterstaining is shown in blue.

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC- 173, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, APC, Catalog #60097AZ).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, Biotin, Catalog #60097BT) followed by SAV APC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, FITC, Catalog #60097FI).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, PE, Catalog #60097PE).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE
Catalog #
60071PE, 60071PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin
Catalog #
60071BT.1, 60071BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC
Catalog #
60071AZ, 60071AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173
Catalog #
60071, 60071.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC
Catalog #
60071FI.1, 60071FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488
Catalog #
60071AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE
Catalog #
60071PE, 60071PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin
Catalog #
60071BT.1, 60071BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC
Catalog #
60071AZ, 60071AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173
Catalog #
60071, 60071.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC
Catalog #
60071FI.1, 60071FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488
Catalog #
60071AD
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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