Mobilized Human Peripheral Blood CD34+ Cells, Frozen
Primary human cells, frozen
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Overview
Donors are mobilized with:
• G-CSF: a maximum of 10 µg/kg/day of granulocyte colony-stimulating factor for 3 - 5 days prior to collection (Catalog #70060)
• Plerixafor: a maximum of 0.24 mg/kg plerixafor for 1 day prior to collection (Catalog #70075)
• G-CSF and Plerixafor: a maximum of 10 µg/kg/day of G-CSF for 3 - 5 days prior to collection and a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection (Catalog #70073)
Isolated from leukapheresis samples using positive immunomagnetic separation techniques and cryopreserved in serum-free medium, cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above, after selecting from the product options. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.
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• 10% dimethyl sulfoxide (DMSO)
Data Figures

Figure 1. Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells Generate Hematopoietic Colonies in CFU Assays
Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells (Catalog #70060) were thawed, plated at a concentration of 5 x 10^2 viable cells/dish and cultured in MethoCult™ Optimum Medium (Catalog #04034) for 14 days. Shown are colonies produced by hematopoietic progenitor cells in the sample, imaged with STEMvision™.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (13)
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.