Mobilized Human Peripheral Blood Mononuclear Cells, Frozen
Primary human cells, frozen
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Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen -
Label for Mobilized Human Peripheral Blood Mononuclear Cells, Frozen
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Overview
Primary human mononuclear cells (MNCs) were isolated from mobilized peripheral blood (PB) leukapheresis samples using density gradient separation or red blood cell lysis. PB was collected using acid citrate dextrose solution A (ACDA) as the anticoagulant. Donors were mobilized with:
• Catalog #70049: a maximum of 10 µg/kg/day of granulocyte colony-stimulating factor (G-CSF) for 3 - 5 days prior to collection
• Catalog #70074: a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection
• Catalog #70072: a maximum of 10 µg/kg/day of G-CSF for 3 - 5 days prior to collection and a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection
Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.
Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.
Browse our Frequently Asked Questions (FAQs) on Primary Cells.
• Catalog #70049: a maximum of 10 µg/kg/day of granulocyte colony-stimulating factor (G-CSF) for 3 - 5 days prior to collection
• Catalog #70074: a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection
• Catalog #70072: a maximum of 10 µg/kg/day of G-CSF for 3 - 5 days prior to collection and a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection
Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.
Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.
Browse our Frequently Asked Questions (FAQs) on Primary Cells.
Contains:
• CryoStor® CS10
Subtype:
Frozen
Cell Type:
Hematopoietic Stem and Progenitor Cells; Mononuclear Cells
Species:
Human
Cell and Tissue Source:
Peripheral Blood
Donor Status:
Normal
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Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
70049, 70049.1, 70049.2, 70049.3, 70049.4
All
70072, 70072.1, 70072.2, 70072.3, 70072.4
All
70074, 70074.1, 70074.2, 70074.3, 70074.4
All
Educational Materials
(11)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Figure 1. Cryopreserved G-CSF Mobilized Human Peripheral Blood Mononuclear Cells Generate Hematopoietic Colonies in CFU Assays
Cryopreserved G-CSF Mobilized Human Peripheral Blood Mononuclear Cells (Catalog #70049) were thawed, plated at a concentration of 1 x 10^4 viable cells/dish and cultured in MethoCult™ Optimum Medium (Catalog #04034) for 14 days. Shown are colonies produced by hematopoietic progenitor cells within the sample, imaged with STEMvision™.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.