Human Recombinant EGF, ACF

Epidermal growth factor, animal component-free

Human Recombinant EGF, ACF

Epidermal growth factor, animal component-free

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Epidermal growth factor, animal component-free
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Overview

Epidermal growth factor (EGF) is characterized by high-affinity binding to various EGF receptors (EGFRs) and the production of mitogenic responses (Carpenter & Cohen). EGF promotes EGFR dimerization, resulting in activation of downstream pathways including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signaling. EGF is secreted by the gut-associated salivary and Brunner’s glands, is found in a variety of body fluids, and stimulates cell proliferation and differentiation in rodent and neonatal human intestine (Wright et al.). Central nervous system stem cells also proliferate in response to the EGF stimulus (Reynolds & Weiss). This product is animal component-free.
Subtype
Cytokines, Growth Factors
Cell Type
Brain Tumor Stem Cells, Endoderm, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Mesenchymal Stem and Progenitor Cells, Mesoderm, PSC-Derived, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons, Pluripotent Stem Cells, Prostate Cells
Species
Human
Area of Interest
Epithelial Cell Biology, Neuroscience, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant EGF, ACF was tested by its ability to promote the proliferation of BALB/c 3T3 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.0459 ng/mL. (B) 1 μg of Human Recombinant EGF, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78136.1, 78136.2, 78136
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78136.1, 78136.2, 78136
Lot #
All
Language
English