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CFDA-SE (5(6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE. Upon diffusion into the cell, intracellular esterases cleave the acetate group to generate CFSE, which interacts with cellular amines via its succinimidyl groups to generate a highly fluorescent green dye that is impermeant to the cell membrane. CFDA-SE is frequently used in cell proliferation assays, as it is partitioned approximately equally between the progeny so that cell division can be followed as a successive halving of the fluorescence intensity through multiple generational divisions. CFDA-SE is also used for motility assays and in vivo cell tracking experiments. CFDA-SE-labeled cells can be detected with any instrument/filter set compatible with fluorescein detection.
(A) Flow cytometry analysis of Sp2/0 mouse myeloma cells labeled with CFDA-SE and analyzed by flow cytometry after being cultured for 0, 24, and 48 hours (filled histograms). Solid line histogram (control) shows unlabeled cells analyzed after 48 hours of cell culture. (B) Chemical structure of CFDA-SE.
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