Resazurin (Sodium Salt)

Cell proliferation and viability dye

Resazurin (Sodium Salt)

Cell proliferation and viability dye

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Cell proliferation and viability dye
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Overview

Resazurin (7-hydroxy-10-oxidophenoxazin-10-ium-3-one, sodium) is a blue fluorogenic dye used as a redox indicator in cell viability and proliferation assays for bacteria, yeast or mammalian cells. The blue form of the dye is irreversibly reduced by enzymes in viable cells to generate a highly red-fluorescent product, Resazurin, which exhibits an emission maximum at ~580 nm and can be detected by flow cytometry, fluorescence microscopy, and high-throughput screening methods. Resazurin is minimally toxic to living cells, making it suitable for use in long-term cell culture. The dye has also been used to assay L-glutamate and to measure the metabolic activity of mitochondria.
Cell Type
Other
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Flow Cytometry
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
CAS Number
62758-13-8
Chemical Formula
C₁₂H₆NO₄ · Na

Data Figures

Resazurin (Sodium Salt)

Figure 1. Resazurin (Sodium Salt)

Chemical structure of Resazurin (Sodium Salt).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
75005
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
75005
Lot #
All
Language
English

Resources and Publications

Educational Materials (1)