CFDA-SE

Cell proliferation and tracking dye
CFDA-SE

Cell proliferation and tracking dye

10 mg
Catalog # 75003
117 USD

Overview

CFDA-SE (5(6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE. Upon diffusion into the cell, intracellular esterases cleave the acetate group to generate CFSE, which interacts with cellular amines via its succinimidyl groups to generate a highly fluorescent green dye that is impermeant to the cell membrane. CFDA-SE is frequently used in cell proliferation assays, as it is partitioned approximately equally between the progeny so that cell division can be followed as a successive halving of the fluorescence intensity through multiple generational divisions. CFDA-SE is also used for motility assays and in vivo cell tracking experiments. CFDA-SE-labeled cells can be detected with any instrument/filter set compatible with fluorescein detection.
Cell Type
Other
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
CAS Number
150347-59-4
Chemical Formula
C₂₉H₁₉NO₁₁
Molecular Weight
557.5 g/mol

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
CFDA-SE
Catalog #
75003
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
CFDA-SE
Catalog #
75003
Lot #
All
Language
English

Educational Materials (1)

Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Data and Publications

Data

(A) Flow cytometry analysis of Sp2/0 mouse myeloma cells labeled with CFDA-SE and analyzed by flow cytometry after being cultured for 0, 24, and 48 hours (filled histograms). Solid line histogram (control) shows unlabeled cells analyzed after 48 hours of cell culture. (B) Chemical structure of CFDA-SE.

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