Trypsin-EDTA (0.05%)

Enzymatic cell dissociation reagent

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Trypsin-EDTA (0.05%)

Enzymatic cell dissociation reagent

500 mL
Catalog #07910
126 USD

Overview

Trypsin-EDTA (0.05%) can be used to achieve a single-cell suspension of human embryonic stem (ES) cells or human induced pluripotent stem (iPS) cells for flow cytometry or cloning assays. This is a gentler version than the standard concentration (0.25%), and has applications to many other cell culture systems in addition to human ES cells and iPS cells.
Contains:
• 0.5 g/L Porcine trypsin
• Hanks' Balanced Salt Solution (without Ca++ and Mg++)
• 0.2 g/L EDTA•4Na
Subtype:
Enzymatic
Species:
Human; Mouse; Non-Human Primate; Other; Rat

Scientific Resources

Data and Publications

Publications

(1)
Nature Communications 2015 DEC

Intermediate DNA methylation is a conserved signature of genome regulation

Elliott G et al.

Abstract

The role of intermediate methylation states in DNA is unclear. Here, to comprehensively identify regions of intermediate methylation and their quantitative relationship with gene activity, we apply integrative and comparative epigenomics to 25 human primary cell and tissue samples. We report 18,452 intermediate methylation regions located near 36% of genes and enriched at enhancers, exons and DNase I hypersensitivity sites. Intermediate methylation regions average 57% methylation, are predominantly allele-independent and are conserved across individuals and between mouse and human, suggesting a conserved function. These regions have an intermediate level of active chromatin marks and their associated genes have intermediate transcriptional activity. Exonic intermediate methylation correlates with exon inclusion at a level between that of fully methylated and unmethylated exons, highlighting gene context-dependent functions. We conclude that intermediate DNA methylation is a conserved signature of gene regulation and exon usage.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.