Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3

Mouse monoclonal IgG2a antibody against human, mouse, rat beta­tubulin III

More Views

From: 121 USD

Options

* Required Fields

Catalog # (Select a product)
Mouse monoclonal IgG2a antibody against human, mouse, rat beta­tubulin III
From: 121 USD

Overview

The 2G10-TB3 antibody reacts with beta-tubulin III, an ~50 - 55 kDa structural protein that is a constituent of tubulin. Tubulin is the major component of microtubules within the cytoskeleton and is assembled from heterodimers of alpha- and beta-tubulin subunits. The beta III isoform of tubulin, also known as neuron-specific class III beta-tubulin, is expressed primarily in neurons and is widely used as a marker to distinguish neurons from other cell types. Beta-tubulin III contributes to microtubule formation in neuronal cell bodies and axons, a function involving GTP binding, and plays roles in axonal transport, neuronal cell proliferation, and differentiation. It is highly expressed in several types of cancer and is a predictive and prognostic marker for various tumors, for example, being found in neoplastic but not in normal glial cells. The 2G10-TB3 antibody is expected to recognize all mammalian homologs of beta-tubulin III and the epitope has reportedly been mapped to the extreme C-terminal amino acids, EAQGPK.
Subtype:
Primary Antibodies
Target Antigen:
Beta-Tubulin III
Alternative Names:
Class 3 beta-tubulin, class III beta-tubulin, MC1R, neuron-specific class 3 beta-tubulin, neuron-specific class III beta-tubulin, TUBB 3, TUBB3, tubulin beta 3, tubulin beta 4, tubulin beta III
Reactive Species:
Human; Mouse; Rat
Conjugation:
Unconjugated
Host Species:
Mouse
Cell Type:
Neural Cells, PSC-Derived; Neurons
Application:
Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blotting
Area of Interest:
Neuroscience
Clone:
2G10-TB3
Gene ID:
10381
Isotype:
IgG2a, kappa

Scientific Resources

Educational Materials

(3)

Data and Publications

Data

Figure 1. Data for Unconjugated

(A) E18 cortical rat neurons were cultured using NeuroCult™ SM1 Neuronal Culture Kit on poly-lysine-coated glass coverslips, then fixed and labeled with Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3, followed by donkey anti-mouse IgG, Alexa Fluor® 488. Nuclei were counterstained with DAPI. Inset shows cells labeled with a mouse IgG2a, kappa isotype control antibody followed by donkey anti-mouse IgG, Alexa Fluor® 488, and counterstained with DAPI. (B) Western blot analysis of denatured/reduced cell lysates with Anti-Beta Tubulin III Antibody, Clone 2G10-TB3. Lane 1, adult rat brain cortical cells; lane 2 (negative control), mouse E13.5 neural progenitor cells cultured with NeuroCult™ Proliferation Kit (Mouse & Rat).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.