STEMdiff™ Pancreatic Progenitor Kit

Serum-free medium for the differentiation of human ES and iPS cells to pancreatic progenitor cells
STEMdiff™ Pancreatic Progenitor Kit

Serum-free medium for the differentiation of human ES and iPS cells to pancreatic progenitor cells

1 Kit
Catalog # 05120
1,030 USD
Required Products
  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. Y-27632 (Dihydrochloride)
    Y-27632

    RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2

  3. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

STEMdiff™ Pancreatic Progenitor Kit is a serum-free medium that supports efficient and reproducible generation of pancreatic progenitor cells from human pluripotent stem cells (hPSCs). Cells are differentiated through four stages: 1) definitive endoderm, 2) primitive gut tube, 3) posterior foregut endoderm, and 4) pancreatic progenitor cells. The differentiated cells express key markers of pancreatic progenitor cells, including PDX-1, NKX6.1 and SOX9 and up-regulation of insulin and glucagon. The resulting pancreatic progenitor cells can be further differentiated to insulin-producing beta cells or other endocrine and exocrine pancreatic cell fates.

STEMdiff™ Pancreatic Progenitor Kit has been optimized for differentiation of cells maintained in mTeSR™1 (Catalog #85850).
Advantages
• Generates functional pancreatic progenitor cells for transplantation into animal models or for further maturation into insulin-producing beta cells
• Efficient and reproducible differentiation of multiple hPSC lines to pancreatic progenitor cells expressing PDX-1, NKX6.1, and SOX9
• Serum-free medium
• Compatible with multiple human ES and iPS cell lines maintained in mTeSR™1
Components
  • STEMdiff™ Endoderm Basal Medium, 100 mL
  • STEMdiff™ Pancreatic Stage 2 - 4 Basal Medium, 265 mL
  • STEMdiff™ Definitive Endoderm Supplement MR (100X), 350 µL
  • STEMdiff™ Definitive Endoderm Supplement CJ (100X), 1.1 mL
  • STEMdiff™ Pancreatic Supplement 2A, 240 µL
  • STEMdiff™ Pancreatic Supplement 2B, 720 µL
  • STEMdiff™ Pancreatic Supplement 3, 720 µL
  • STEMdiff™ Pancreatic Supplement 4, 1.2 mL
Subtype
Specialized Media
Cell Type
Pancreatic Cells, Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Differentiation
Brand
STEMdiff
Area of Interest
Cancer, Disease Modeling, Epithelial Cell Biology, Stem Cell Biology
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet 1
Product Name
STEMdiff™ Pancreatic Progenitor Kit
Catalog #
05120
Lot #
Component #05113: Lot 18D89658 and higher
Language
English
Document Type
Product Information Sheet 2
Product Name
STEMdiff™ Pancreatic Progenitor Kit
Catalog #
05120
Lot #
Component #05113: Lot 18D89658 and higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Pancreatic Progenitor Kit
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Pancreatic Progenitor Kit
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
STEMdiff™ Pancreatic Progenitor Kit
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
STEMdiff™ Pancreatic Progenitor Kit
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
STEMdiff™ Pancreatic Progenitor Kit
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
STEMdiff™ Pancreatic Progenitor Kit
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
STEMdiff™ Pancreatic Progenitor Kit
Catalog #
05120
Lot #
All
Language
English

Educational Materials (10)

Brochure
qPCR Arrays for Cell Characterization
Brochure
Products for Human Pluripotent Stem Cells
Brochure
STEMdiff™ Pancreatic Progenitor Kit
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Wallchart
SnapShot: GI Tract Development
Webinar
Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells
56:31
Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells
Webinar
Understanding Pancreatic Development and Diabetes Using Patient-Specific iPS Cells
50:32
Understanding Pancreatic Development and Diabetes Using Patient-Specific iPS Cells
Webinar
STEMdiff™ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
20:24
STEMdiff™ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
Scientific Poster
Highly Efficient and Reproducible Differentiation of hPSCs to PDX1+ NKX6.1+ Pancreatic Progenitors
Scientific Poster
Reproducible and Efficient Differentiation of Human Pluripotent Stem Cells to Pancreatic Progenitors Using a Novel Serum-Free Medium

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Pancreatic Differentiation of Progenitor Cells into Mature Endocrine and Exocrine Cells

Figure 1. Pancreatic Progenitor Cells can Mature into Endocrine and Exocrine Cells

A) Representative image showing pancreatic progenitor cells expressing PDX-1 (red) and NKX6.1 (green). Yellow staining indicates co‑expression of both markers in the majority of cells as is observed in the developing human pancreas.1 (B, C) Cells transplanted into mice can mature into endocrine and exocrine cells.
B) shows endocrine clusters expressing synaptophysin (red) surrounded by ductal structures expressing CK-19 (green).
C) Shows islet-like structures containing monohormonal cells that individually express insulin (red), glucagon (green) or somatostatin (blue). Data in (B, C) are from the laboratory of Dr. Timothy J. Kieffer (University of British Columbia, Vancouver, Canada).

A. The STEMdiff™ Pancreatic Progenitor Kit Functions Efficiently Across Multiple hPSC Lines
B. The STEMdiff™ Pancreatic Progenitor Kit Functions Efficiently Across Multiple hPSC Lines

Figure 2. The STEMdiff™ Pancreatic Progenitor Kit Functions Efficiently Across Multiple hPSC Lines

PDX-1 and NKX6.1 expression measured in pancreatic progenitor cells derived from four different hPSC lines (H1, H9, WLS-4D1 and WLS-1C) at the end of Stage 4. (A) Representative flow cytometry plots show PDX-1 and NKX6.1 co-expression in differentiated H9 cells. (B) Quantitative data for PDX-1/NKX6.1 co-expression in two human ES (H1 and H9) and two human iPS (WLS-4D1 and WLS-1C) cell lines (n = 5-10 per cell line). Data are plotted as individual points representing the mean of duplicates within a single experiment. The horizontal line represents the mean of all experiments, with error bars indicating the standard error of the mean (SEM). The average efficiency of pancreatic progenitor differentiation ranges from 61.5% to 77.7% depending on the cell line.

Gene Expression Profile is Indicative of Transition to Pancreatic Progenitor Cells

Figure 3. Gene Expression Profile is Indicative of Transition to Pancreatic Progenitor Cells

Gene expression profile at the end of each stage of differentiation for key markers of pancreatic progenitor cells. Expression was normalized to 18S ribosomal RNA and TATA Binding Protein (TBP). Data are the mean ± SEM for 3 - 5 experiments. Expression pattern is consistent with published data.²
 
1. Riedel M et al. (2012) Immunohistochemical characterization of cells co-producing insulin and glucagon in the developing human pancreas. Diabetologia 55(2): 372-81.
2. Rezania A et al. (2014) Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells. Nat Biotechnol 32(11): 1121-33.

Density plots and quantitative analysis showing PDX-1 and NKX6.1 expression in cells cultured in mTeSR™1 or mTeSR™ Plus, following 5 days of differentiation using the STEMdiff™ Pancreatic Progenitor Kit.

Figure 4. Generation of Pancreatic Progenitors from hPSCs Maintained in mTeSR™ Plus

(A) Representative density plots showing PDX-1 and NKX6.1 expression in cells cultured in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds), following differentiation using the STEMdiff™ Pancreatic Progenitor Kit. (B) Quantitative analysis of pancreatic progenitor formation in multiple hPS (H9, STiPS-M001, WLS-1C) cell lines maintained with mTeSR™1 or mTeSR™ Plus as measured by co-expression of PDX-1 and NKX6.1. Data are expressed as the mean percentage of cells (± SEM) expressing both markers; n=3.

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