STEMdiff™ Pancreatic Progenitor Kit

Serum-free medium for the differentiation of human ES and iPS cells to pancreatic progenitor cells

STEMdiff™ Pancreatic Progenitor Kit

Serum-free medium for the differentiation of human ES and iPS cells to pancreatic progenitor cells

STEMdiff™ Pancreatic Progenitor Kit
1 Kit
1,030 USD
Catalog # 05120

Serum-free medium for the differentiation of human ES and iPS cells to pancreatic progenitor cells

Product Advantages


  • Generates functional pancreatic progenitor cells for transplantation into animal models or for further maturation into insulin-producing beta cells

  • Efficient and reproducible differentiation of multiple hPSC lines to pancreatic progenitor cells expressing PDX-1, NKX6.1, and SOX9

  • Serum-free medium

  • Compatible with multiple human ES and iPS cell lines maintained in mTeSR™1

What's Included

  • STEMdiff™ Endoderm Basal Medium, 100 mL
  • STEMdiff™ Pancreatic Stage 2 - 4 Basal Medium, 265 mL
  • STEMdiff™ Definitive Endoderm Supplement MR (100X), 350 µL
  • STEMdiff™ Definitive Endoderm Supplement CJ (100X), 1.1 mL
  • STEMdiff™ Pancreatic Supplement 2A, 240 µL
  • STEMdiff™ Pancreatic Supplement 2B, 720 µL
  • STEMdiff™ Pancreatic Supplement 3, 720 µL
  • STEMdiff™ Pancreatic Supplement 4, 1.2 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Product Information Sheet.

Overview

STEMdiff™ Pancreatic Progenitor Kit is a serum-free medium that supports efficient and reproducible generation of pancreatic progenitor cells from human pluripotent stem cells (hPSCs). Cells are differentiated through four stages: 1) definitive endoderm, 2) primitive gut tube, 3) posterior foregut endoderm, and 4) pancreatic progenitor cells. The differentiated cells express key markers of pancreatic progenitor cells, including PDX-1, NKX6.1 and SOX9 and up-regulation of insulin and glucagon. The resulting pancreatic progenitor cells can be further differentiated to insulin-producing beta cells or other endocrine and exocrine pancreatic cell fates.

STEMdiff™ Pancreatic Progenitor Kit has been optimized for differentiation of cells maintained in mTeSR™1 (Catalog #85850).
Subtype
Specialized Media
Cell Type
Pancreatic Cells, Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Differentiation
Brand
STEMdiff
Area of Interest
Cancer, Disease Modeling, Epithelial Cell Biology, Stem Cell Biology
Formulation
Serum-Free

Data Figures

Pancreatic Differentiation of Progenitor Cells into Mature Endocrine and Exocrine Cells

Figure 1. Pancreatic Progenitor Cells can Mature into Endocrine and Exocrine Cells

A) Representative image showing pancreatic progenitor cells expressing PDX-1 (red) and NKX6.1 (green). Yellow staining indicates co‑expression of both markers in the majority of cells as is observed in the developing human pancreas.1 (B, C) Cells transplanted into mice can mature into endocrine and exocrine cells.
B) shows endocrine clusters expressing synaptophysin (red) surrounded by ductal structures expressing CK-19 (green).
C) Shows islet-like structures containing monohormonal cells that individually express insulin (red), glucagon (green) or somatostatin (blue). Data in (B, C) are from the laboratory of Dr. Timothy J. Kieffer (University of British Columbia, Vancouver, Canada).

A. The STEMdiff™ Pancreatic Progenitor Kit Functions Efficiently Across Multiple hPSC Lines
B. The STEMdiff™ Pancreatic Progenitor Kit Functions Efficiently Across Multiple hPSC Lines

Figure 2. The STEMdiff™ Pancreatic Progenitor Kit Functions Efficiently Across Multiple hPSC Lines

PDX-1 and NKX6.1 expression measured in pancreatic progenitor cells derived from four different hPSC lines (H1, H9, WLS-4D1 and WLS-1C) at the end of Stage 4. (A) Representative flow cytometry plots show PDX-1 and NKX6.1 co-expression in differentiated H9 cells. (B) Quantitative data for PDX-1/NKX6.1 co-expression in two human ES (H1 and H9) and two human iPS (WLS-4D1 and WLS-1C) cell lines (n = 5-10 per cell line). Data are plotted as individual points representing the mean of duplicates within a single experiment. The horizontal line represents the mean of all experiments, with error bars indicating the standard error of the mean (SEM). The average efficiency of pancreatic progenitor differentiation ranges from 61.5% to 77.7% depending on the cell line.

Gene Expression Profile is Indicative of Transition to Pancreatic Progenitor Cells

Figure 3. Gene Expression Profile is Indicative of Transition to Pancreatic Progenitor Cells

Gene expression profile at the end of each stage of differentiation for key markers of pancreatic progenitor cells. Expression was normalized to 18S ribosomal RNA and TATA Binding Protein (TBP). Data are the mean ± SEM for 3 - 5 experiments. Expression pattern is consistent with published data.²
 
1. Riedel M et al. (2012) Immunohistochemical characterization of cells co-producing insulin and glucagon in the developing human pancreas. Diabetologia 55(2): 372-81.
2. Rezania A et al. (2014) Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells. Nat Biotechnol 32(11): 1121-33.

Density plots and quantitative analysis showing PDX-1 and NKX6.1 expression in cells cultured in mTeSR™1 or mTeSR™ Plus, following 5 days of differentiation using the STEMdiff™ Pancreatic Progenitor Kit.

Figure 4. Generation of Pancreatic Progenitors from hPSCs Maintained in mTeSR™ Plus

(A) Representative density plots showing PDX-1 and NKX6.1 expression in cells cultured in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds), following differentiation using the STEMdiff™ Pancreatic Progenitor Kit. (B) Quantitative analysis of pancreatic progenitor formation in multiple hPS (H9, STiPS-M001, WLS-1C) cell lines maintained with mTeSR™1 or mTeSR™ Plus as measured by co-expression of PDX-1 and NKX6.1. Data are expressed as the mean percentage of cells (± SEM) expressing both markers; n=3.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Safety Data Sheet 1
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Catalog #
05120
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Catalog #
05120
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

We get a really great efficiency of differentiation in several pluripotent stem cell lines determined by expression of key markers: upregulation of PDX1, SOX9, FOXA2 and GATA4, and down-regulation of Sox17. We can generate these pancreatic progenitor cells reproducibly and efficiently; variability within the protocol is low.

Drs. Jamie Trott and Ray Dunn, A*STAR Institute of Medical Biology (IMB), Singapore

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.