qPCR is used to determine changes in steady-state mRNA levels of gene expression across multiple samples, generally normalized to the relative expression of internal control genes. Gene-specific primers amplify target sequences within cDNA pools reverse-transcribed from mRNA and, as with TaqMan® technology, hybridized sequence-specific probes provide a fluorescent signal from the 5' exonuclease activity of Taq DNA polymerase. The accumulation rate of the fluorescent signal is used to quantify the sample cDNA, and thereby the amount of mRNA in the original cell lysate.
This qPCR array contains validated primers and probes for detection of 90 genes whose expression is correlated with undifferentiated hPSCs or their derivatives undergoing the early stages of differentiation, as well as six endogenous (housekeeping) control genes. TBP (TATA box-binding protein) qPCR Control Template is provided as a synthetic DNA positive control. Additional gene information and a qPCR analysis tool are available at www.stemcell.com/qPCRanalysis
- Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array (Catalog #07515)
- hPSC Trilineage qPCR Array, 1 x 384-well plate
- TBP qPCR Control Template, 1 vial
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Data and Publications
Figure 1. Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array Plate Configuration