Human Recombinant R-Spondin-1

R-Spondin-1
Catalog #
78213_C
R-Spondin-1
From: 240 USD

Overview

R-Spondin-1 (RSPO1) is the prototype member of the R-Spondin (RSPO) protein subfamily of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins (Chen et al.; Kamata et al.; Kazanskaya et al.; Kim et al.). Although unable to initialize signaling, RSPO family members are potent enhancers of WNT signaling (Cruciat & Niehrs; de Lau et al.; Kamata et al.; Kazanskaya et al.). They are characterized by a TSR-1 domain, a carboxy-terminal region with positively charged amino acids, and two N-terminal furin-like cysteine-rich repeats (Glinka et al.; Kazanskaya et al.). R­-Spondin-1 activates β­-catenin signaling via the WNT signaling cascade and by indirectly increasing low-density lipoprotein receptor-related protein (LRP6) on the cell surface. It does this by binding leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), and competing with WNT antagonist DKK­1 for binding to the WNT co­receptors, Kremen and LRP­6, which reduces DKK­1-­mediated internalization of LRP6 (Binnerts et al.). RSPO1 is involved in a wide range of pleiotropic roles during embryogenesis, it is required for the specification of hematopoietic stem cells, and it has been shown to be important in the growth, survival, and migration of ovarian cancer cells (Cruciat & Niehrs; de Lau et al.; Genthe & Clements; Liu et al.).
Subtype
Cytokines
Alternative Names
hRspo1, Roof plate-specific Spondin-1
Cell Type
Endoderm, PSC-Derived, Epithelial Cells, Hematopoietic Stem and Progenitor Cells, Intestinal Cells, Mesoderm, PSC-Derived, Other, Pluripotent Stem Cells
Species
Human
Area of Interest
Epithelial Cell Biology, Stem Cell Biology
Molecular Weight
25.6 kDa
Purity
≥ 95%

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant R-Spondin-1
Catalog #
78213, 78213.1, 78213.2, 78213.3
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant R-Spondin-1
Catalog #
78213, 78213.1, 78213.2, 78213.3
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant R-Spondin-1 was tested by its ability to induce luciferase activity in a WNT reporter HEK-293 cell line in the presence of mouse Wnt-3a. Luciferase activity was measured using a luminometric assay method. The EC50 is defined as the effective concentration of the growth factor at which luciferase activity is at 50% of maximum. The EC50 in the above example is 36.4 ng/mL. (B) Human Recombinant R-Spondin-1 was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant R-Spondin-1 has a predicted molecular mass of 25.6 kDa.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.