hPSC-Derived Neural Cell Research

Human pluripotent stem cells (hPSCs), including human embryonic stem cells (ES) cells and induced pluripotent stem cells (iPS) cells are self-renewing cells that have the potential to develop into cells of all three germ layers. See MorehPSCs are a valuable tool for researchers aiming to model human neurological development and disease. By generating iPS cells from patient samples, disease-specific neural stem cells, neurons and glia can be obtained. The first step in your hPSC to neural workflow is the neural induction of hPSCs to generate neural progenitor cells (NPCs). To achieve reproducible and efficient neural induction, use the defined and serum-free STEMdiff™ Neural Induction Medium. This medium is compatible with both embryoid body and monolayer neural induction protocols. The embryoid body protocol utilizes AggreWell™800 plates to form uniform neural aggregates, which, when replated are characterized by the presence of morphologically distinct neural rosette clusters that can be isolated for further experiments using STEMdiff™ Neural Rosette Selection Reagent.

As an alternative to neural induction, highly pure cryopreserved hPSC-derived NPCs are available from various cell lines. These NPCs can be expanded with Neural Progenitor Medium 2 or differentiated to neurons and glia using STEMdiff™ differentiation and maturation kits for mixed neurons, dopaminergic neurons and astrocytes.

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Cell Culture Media and Supplements (47)

Cell Dyes and Detection Assay Kits (42)

Cell Storage Media (41)

Cultureware and General Supplies (43)

  • AggreWell™400|27845

    AggreWell™

    Easy and reproducible production of embryoid bodies and cancer spheroids
  • AggreWell™800|34811

    AggreWell™800

    Easy and reproducible production of embryoid bodies and tumor spheroids
  • 37 µm Reversible Strainer, small|27215

    37 µm Reversible Strainer

    Reversible 37 µm strainer for filtration and isolation of cellular aggregates, including embryoid bodies and spheroids

Tissue and Cell Culture Dissociation Reagents (43)

Antibodies (43)

Cytokines (42)

Small Molecules (49)

  • CHIR99021

    CHIR99021

    WNT pathway activator; Inhibits GSK3
  • EC23

    EC23

    Retinoid pathway activator; Activates retinoic acid receptor (RAR)
  • SAG

    SAG

    Hedgehog pathway activator; Activates Smoothened (SMO)
  • TWS119

    TWS119

    WNT pathway activator; Inhibits GSK3β

Buffers and Solutions (42)

Primary and Cultured Cells (41)

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