Trypsin-EDTA (0.05%)

Enzymatic cell dissociation reagent

Trypsin-EDTA (0.05%)

Enzymatic cell dissociation reagent

From: 176 USD
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Enzymatic cell dissociation reagent
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Product Advantages


  • Generate single-cell suspenstions of human ES and iPS cells for downstream assays with a gentle, low concentration Trypsin-EDTA solution.

Overview

Generate single-cell suspensions of human embryonic stem cells (ES) or human induced pluripotent stem (iPS) cells for flow cytometry or cloning assays with Trypsin-EDTA (0.05%). EDTA weakens cell-cell adhesion, which improves trypsin's access to hydrolysis-targeted peptide bonds, and enhances detachment of adherent cells. This is a gentler version than the standard concentration (0.25%; Catalog #07901), and has applications to many other cell culture systems in addition to human ES cells and iPS cells.
Contains
• 0.5 g/L Porcine trypsin
• Hanks' Balanced Salt Solution (without Ca++ and Mg++)
• 0.2 g/L EDTA•4Na
Subtype
Enzymatic
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Cell Culture

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
07910
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
07910
Lot #
All
Language
English

Resources and Publications

Publications (1)

Intermediate DNA methylation is a conserved signature of genome regulation Elliott G et al. Nature Communications 2015 DEC

Abstract

The role of intermediate methylation states in DNA is unclear. Here, to comprehensively identify regions of intermediate methylation and their quantitative relationship with gene activity, we apply integrative and comparative epigenomics to 25 human primary cell and tissue samples. We report 18,452 intermediate methylation regions located near 36% of genes and enriched at enhancers, exons and DNase I hypersensitivity sites. Intermediate methylation regions average 57% methylation, are predominantly allele-independent and are conserved across individuals and between mouse and human, suggesting a conserved function. These regions have an intermediate level of active chromatin marks and their associated genes have intermediate transcriptional activity. Exonic intermediate methylation correlates with exon inclusion at a level between that of fully methylated and unmethylated exons, highlighting gene context-dependent functions. We conclude that intermediate DNA methylation is a conserved signature of gene regulation and exon usage.