Isolate Virtually Any Cell Type Using EasySep™ or EasySep™ Release Indirect Selection Kits

Can't find the right kit to isolate your cells? STEMCELL Technologies offers multiple indirect selection kits to isolate virtually any cell type using EasySep™ or EasySep™ Release. Use any biotinylated, FITC-, PE- or APC-conjugated antibody to label your cells of interest. The labeled cell suspension is then mixed with the appropriate EasySep™ reagents according to the EasySep™ protocol to obtain highly purified cells.
Positive Selection Kit Catalog #
Human Mouse Any Species
EasySep™ Biotin 17663 17665 17683
EasySep™ FITC 17662 17668 17682
EasySep™ PE 17664 17666 17684
EasySep™ APC 17661 17667 17681
EasySep™ Release Biotin 17653 17655 N/A
EasySep™ Release PE 17654 17656 N/A


Please refer to the following tips to ensure optimal isolation of your cell type of interest:

  1. Ensure cells are in single cell suspension.
  2. Add species-specific blocker to cell suspension to minimize non-specific binding. For more information on using Fc receptor (FcR) blockers, please refer to our Technical Tip: Importance of Using an FcR Blocker During Cell Separation Procedures.
  3. We recommend titrating your primary Biotin/FITC/PE/APC-conjugated antibody. The desired final concentration will be the lowest concentration of your primary antibody that gives the greatest fluorescence intensity shift by flow cytometry (for best results, aim to achieve 2 log staining intensity above background). We suggest using a final concentration of primary antibody between 0.3 - 3.0 µg/mL of cells, the optimal concentration will vary depending on the starting frequency of your cells of interest. 
If the starting percentage of your desired cell type is:

  • <10%, use a final concentration of antibody between 0.3 - 0.5 µg/mL
  • 10-40%, use a final concentration of antibody between 0.5 - 1.0 µg/mL
  • 40-60%, use a final concentration of antibody between 1.0 - 2.0 µg/mL
  • >60% use a final concentration of antibody between 2.0 - 3.0 µg/mL *

*NOTE: For samples where the start percentage of desired cells is greater than 60%, it may be advantageous to reduce the number of magnetic separations in order to improve recovery.

  1. Some antibodies used to assess purity of the isolated cells will be blocked by the initial selection antibody, in which case you should stain with an alternate antibody. You could use an antibody for a different cell surface antigen (i.e. assess purity using alternate marker expressed on your target cells), or use a fluorochrome-conjugated antibody that will recognize the primary antibody (i.e. a goat anti-mouse antibody, such as GAM-FITC).
For further assistance and information, please contact techsupport@stemcell.com.