SepMate™

Hassle-Free PBMCs in Just 15 Minutes

SepMate™ is an innovative new tube that enables consistent and hassle-free PBMC isolation in just 15 minutes. The SepMate™ insert allows users to quickly layer blood over the density gradient medium (e.g. Ficoll- Paque®, Lymphoprep™, etc.), and prevents the layers from mixing. Centrifugation is performed with the brake on, after which isolated PBMCs are simply poured off into a fresh tube. Read a SepMate™ review on Biocompare.
RosetteSep™: Cell Isolation Directly From Whole Blood Without Columns or Magnets

How To Use SepMate™

This technical video provides a simple procedure for isolating peripheral blood mononuclear cells (PBMCs) from whole blood using SepMate™. It also demonstrates how to combine SepMate™ with RosetteSep™ to isolate highly purified cell subsets in just 25 minutes.

Why Use SepMate™?

  • Say goodbye to slow and laborious sample layering over the density gradient medium.
  • Centrifuge for just 10 minutes with the brake on and simply pour off PBMCs into a new tube.
  • Minimize variability between users.
  • Can be combined with RosetteSep™ to isolate specific cell subsets.
SepMate™ is manufactured following current Good Manufacturing Practices (cGMP) and tubes are sterilized by irradiation. SepMate™ is registered as an In Vitro Diagnostic (IVD) device in select regions for the isolation of mononuclear cells from whole blood or bone marrow by density gradient centrifugation. In regions where SepMate™ is not registered as an IVD device it is available as a Research Use Only (RUO) product and is not intended for diagnostic or therapeutic uses.

STEMCELL operates a Quality Management System which complies with the requirements of ISO 13485:2003 in the manufacture of SepMate™ tubes.

SepMate™-15

Blood Volume Processed

0.5 - 5 mL

Unit Size

100 tubes
500 tubes

Accessory Products

SepMate™-50

Blood Volume Processed

4 -17 mL

Unit Size

100 tubes
500 tubes

Accessory Products

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SepMate™-15

Product Format
Discontinued Catalog #
New SepMate™ IVD Catalog #
New SepMate™ RUO Catalog #
20 tube pack
15415
No Longer Available
No Longer Available
100 tube pack
15420
500 tube pack
15425
1000 tube pack
15430
No Longer Available
No Longer Available


SepMate™-50

Product Format
Discontinued Catalog #
New SepMate™ IVD Catalog #
New SepMate™ RUO Catalog #
20 tube pack
15450
No Longer Available
No Longer Available
100 tube pack
15460
500 tube pack
15465
1000 tube pack
15470
No Longer Available
No Longer Available

Key Applications

Cell Isolation Directly From Whole Blood

The NK Cell Cytoxicity Case Study discusses a publication from Dr. Ajay Jain’s lab at the University of Maryland School of Medicine, which used the RosetteSep™ and SepMate™ system to reduce NK cell isolation time from four hours to a single hour for a 450 mL unit of blood.

The Typical SepMate™ Protocol



Combine SepMate™ and RosetteSep™ to Isolate Specific Cell Subsets from Whole Blood in 25 Minutes

To isolate purified cell subsets from whole blood, simply incubate your sample with the appropriate RosetteSep™ cocktail for 10 minutes prior to pipetting onto density medium in a SepMate™ tube. During centrifugation unwanted leukocytes pellet along with red blood cells, leaving untouched and highly purified target cells at the interface between the plasma and the density gradient medium above the insert. After centrifugation, simply pour highly purified target cells into a new tube. No columns or magnets are necessary, and minimal training is required. Just incubate, spin, and pour off purified cells.

Copyright © 2016 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, AGGREWELL, CELLADHERE, CLONACELL, EASYPLATE, EASYSEP, ENDOCULT, EPICULT, ESCULT, ERYTHROCLEAR, FRESR, MAMMOCULT, MEGACULT, MESENCULT, METHOCULT, MYELOCULT, NEUROCULT, PNEUMACULT, PROSTACULT, RELESR, ROBOSEP, ROSETTESEP, SEPMATE, SMARTDISH, SPINSEP, STEMDIFF, STEMSEP, STEMSPAN, and STEMVISION are trademarks of STEMCELL Technologies Inc. All other trademarks and registered trademarksare the property of their respective holders.While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information.