RoboSep™ - Automated Cell Separation Instruments

Fully Automated Cell Isolation System

Automate your cell processing and isolations using RoboSep™ cell isolation instruments. The RoboSep™-S and RoboSep™-16 instruments offer true walk-away automated cell separation systems that retain the speed and simplicity of EasySep™ cell separation technology, with as little as 5 minutes of hands-on time. The column-free system minimizes sample handling and eliminates the risk of cross-contamination between samples. You can isolate cells from up to 16 samples at once for flow cytometry, functional studies, or other downstream applications. Learn more about the RoboSep™ below and request a demo for your lab.

Why Use RoboSep™ Cell Separation Instruments?

  • Perform sequential or simultaneous cell isolations from up to 16 samples.
  • Isolate virtually any cells from a wide range of sample sources and sizes using positive or negative selection protocols.
  • Minimize sample handling and risk of exposure to dangerous pathogens.
  • Work with our experts to design customized cell separation protocols to isolate cells of interest for special or non-standard applications.
  • Use without daily washing or decontamination.

We like the reliability of the RoboSep™, the minimization/elimination of specimen handling by the tech during subset separation, and the low maintenance of the instrument. These factors are important to us with such a high throughput of samples processed.

Wendy Leong, Senior Clinical Laboratory Scientist

Compare RoboSep™ Cell Isolation Instruments


RoboSep™-S Automated Cell Separation Instrument


Simultaneous Cell Isolations

Up to 4 samples

Sequential Cell Isolation

Up to 4 cell types from the same sample

Sample Volume

Ranges from 250 µL to 8.5 mL per sample

User-Friendly Interface

Use the integrated touchscreen to start a cell separation protocol in as little as 5 minutes

Reagent and Experiment Tracking

Integrated barcode scanner and End-of-Run reports track user, reagent and protocol details

Compact Design with Removable lid

Multiple units fit easily side-by-side in biological safety cabinet or on lab bench


RoboSep™-16 Automated Cell Separation Instrument


Simultaneous Cell Isolations

Up to 16 samples

Sequential Cell Isolation

Up to 4 cell types from 4 different samples

Sample Volume

Ranges from 200 µL to 2 mL per sample

User-Friendly Interface

Use the RoboSep™-16 application on the laptop computer that is provided to start a cell separation protocol in as little as 5 minutes

Reagent and Experiment Tracking

Integrated barcode scanner and End-of-Run reports track user, reagent and protocol details

Reagent Detection

Conductive pipette tips automatically detect EasySep™ reagent and buffer levels and utilize spare reagents when required
Expand Details
Collapse Details

RoboSep™-S: Automated Cell Isolation for Up to 4 Samples

With an easy-to-use interface and fast cell separation protocols, RoboSep™-S fits easily into the workflow of any lab that needs the multi-sample processing capacity, speed, reliability, and convenience of automated cell isolation. Perform simultaneous cell isolations for up to 4 samples or sequential isolation of up to 4 cell types from the same sample. Simply select your protocol, load the samples and reagents, press “Run”, and return in 25 to 60 minutes to collect your purified cells. The compact instrument design allows you to place multiple units side by side in biological safety cabinets or on lab benches to increase cell processing and isolation capacity. Request a demo for your lab.

RoboSep™-S Cell Separation Instrument Tour

RoboSep™-16: Automated Cell Isolation for Up to 16 Samples

With RoboSep™-16, you can perform fully automated cell processing and isolations from a large number of samples. Through an intuitive user interface, the RoboSep™-16 cell separation system simultaneously isolates cells from up to 16 samples with minimal hands on time. By automating all reagent and sample handling steps, and through the use of disposable pipette tips, the RoboSep™-16 rapidly isolates all desired cells while minimizing the risk of sample cross-contamination. Request a demo for your lab.

RoboSep™-16 Cell Separation Instrument Tour

Key Applications

Fully Automated, High Purity Cell Isolation for Chimerism Labs

This technical bulletin describes a method to automate isolation of lymphoid (T and B cells) and myeloid lineages from human whole blood samples for chimerism analysis.

Automated Lymphocyte Isolation for the Flow Cytometry Crossmatch Assay

This technical bulletin presents a case study of how an automated cell separation system can free up valuable technician time. See how you can use RoboSep™ to minimize sample handling and reduce the overall time to obtain cells for the flow cytometric crossmatch assay, all without compromising assay sensitivity.

Sequential Cell Isolation for Chimerism Analysis

This case study describes a method used by the Florida Tissue Typing Laboratory to sequentially isolate B cells, T cells, myeloid cells, and NK cells starting from a single sample of HetaSep™-treated blood for their chimerism analysis.

Ex Vivo Models for HIV Research

New ex vivo models enable a closer approximation of the in vivo environment than traditional in vitro approaches. Here we highlight four publications that employ a variety of ex vivo models to gain deeper insight into the dynamics of HIV infection.

Technical Resources

Watch a series of videos that provide an overview on how to use and maintain your RoboSep™-S automated cell separation instrument.

See all references using RoboSep™.

See References >

Copyright © 2021 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, AGGREWELL, CELLADHERE, CLONACELL, EASYPLATE, EASYSEP, ENDOCULT, EPICULT, ESCULT, ERYTHROCLEAR, FRESR, GLOCELL, MAMMOCULT, MEGACULT, MESENCULT, METHOCULT, MYELOCULT, NEUROCULT, PNEUMACULT, PROSTACULT, RELESR, ROBOSEP, ROSETTESEP, SEPMATE, SMARTDISH, SPINSEP, STEMDIFF, STEMSEP, STEMSPAN, and STEMVISION are trademarks of STEMCELL Technologies Canada Inc. All other trademarks and registered trademarks are the property of their respective holders. While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.