Applications with RoboSep™

Fully Automated Sequential Cell Isolation of Four Different Cell Types from a Single Sample


Many analyses, such as chimerism testing, are often performed on small blood samples (e.g. pediatric samples). As a result, analysis of purified cell subsets requires techniques that can isolate more than one cell type from an undivided starting sample. Here we describe a method used by the Florida Tissue Typing Laboratory to sequentially isolate B cells, T cells, myeloid cells, and NK cells starting from a single sample of HetaSep™-treated blood for their chimerism analysis.


Figure 1. Automated sequential separation of B cells, T cells, myeloid cells, and NK cells from a single sample of HetaSep™-treated blood.


Figure 2. Purity of four different cell types isolated from 18 different samples using RoboSep™ sequential separation.

Data kindly provided by Max Marschner, Supervisor, Florida Hospital Tissue Typing Lab.

Why Use RoboSep™ for Sequential Isolation of Immune Cells?

  • Up to four cell types can be isolated sequentially from a single sample.
  • All isolations are performed during a single machine cycle with minimal “hands-on” time.
  • Cells are handled with single-use disposable tips, never passing through reusable columns or tubing.
  • High cell purity and viability (>95%) with excellent recovery of desired cells can be achieved.
  • Small volumes of blood (0.5 - 4.5 mL) yield sufficient cells to run DNA analysis or other downstream applications.

Fully Automated, Walk-Away Cell Isolation with RoboSep™ Instruments

Streamline your cell isolations using RoboSep™, the fully automated cell isolation platform. The RoboSep™-S and RoboSep™-16 instruments offer true walk-away automation while retaining the speed and simplicity of EasySep™ with as little as 5 minutes of hands-on time.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.