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Applications with RoboSep™

Fully Automated Sequential Cell Isolation of Four Different Cell Types from a Single Sample

Background

Many analyses, such as chimerism testing, are often performed on small blood samples (e.g. pediatric samples). As a result, analysis of purified cell subsets requires techniques that can isolate more than one cell type from an undivided starting sample. Here we describe a method used by the Florida Tissue Typing Laboratory to sequentially isolate B cells, T cells, myeloid cells, and NK cells starting from a single sample of HetaSep™-treated blood for their chimerism analysis.

Methods

Figure 1. Automated sequential separation of B cells, T cells, myeloid cells, and NK cells from a single sample of HetaSep™-treated blood.

Results

Figure 2. Purity of four different cell types isolated from 18 different samples using RoboSep™ sequential separation.

Data kindly provided by Max Marschner, Supervisor, Florida Hospital Tissue Typing Lab.

Why Use RoboSep™ for Sequential Isolation of Immune Cells?

  • Up to four cell types can be isolated sequentially from a single sample.
  • All isolations are performed during a single machine cycle with minimal “hands-on” time.
  • Cells are handled with single-use disposable tips, never passing through reusable columns or tubing.
  • High cell purity and viability (>95%) with excellent recovery of desired cells can be achieved.
  • Small volumes of blood (0.5 - 4.5 mL) yield sufficient cells to run DNA analysis or other downstream applications.

Fully Automated, Walk-Away Cell Isolation with RoboSep™ Instruments

Streamline your cell isolations using RoboSep™ , the fully automated cell isolation platform. The RoboSep™-S and RoboSep™-16 instruments offer true walk-away automation while retaining the speed and simplicity of EasySep™ with as little as 5 minutes of hands-on time.