EasySep™ Release Mouse Biotin Positive Selection Kit

Immunomagnetic positive selection of biotin-conjugated antibody-labeled mouse cells using particle release technology

New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.

EasySep™ Release Mouse Biotin Positive Selection Kit

Immunomagnetic positive selection of biotin-conjugated antibody-labeled mouse cells using particle release technology

From: 904 USD
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Immunomagnetic positive selection of biotin-conjugated antibody-labeled mouse cells using particle release technology
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Product Advantages


  • Highly purified cells labeled with biotinylated antibodies isolated from mouse tissue in less than 40 minutes

  • No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

  • EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655)
    • EasySep™ Biotin Positive Cocktail, 0.5 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 1 mL
    • EasySep™ Release Buffer (Concentrate), 3 x 1 mL
    • EasySep™ Mouse FcR Blocker (Catalog #18731), 0.5 mL

Overview

Easily isolate highly purified and magnetic particle-free cells labeled with biotin antibodies from mouse splenocytes, using immunomagnetic positive selection, with the EasySep™ Release Mouse Biotin Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.


This EasySep™ positive selection procedure involves labeling cells with antibody complexes recognizing biotin and magnetic particles called EasySep™ Releasable RapidSpheres™. Unlike traditional magnetic particles that stay bound to the target cells, RapidSpheres™ have a releasable feature. Desired cells are first labeled with antibodies and these specialized magnetic particles, before being separated, without columns, using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, biotin-antibody labeled cells using a release agent. Following magnetic cell isolation in less than 40 minutes with this EasySep™ Release kit, the desired cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Antibody complexes remain bound to the surface of the desired cells and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including those for culture media, supplements, antibodies, and more.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ Magnet (Catalog #18102)
• EasyEights™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Innate Lymphoid Cells, Lymphocytes, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Typical EasySep™ Release Mouse Biotin Positive Selection Kit Cell Isolation Profile from PBMCs

Figure 1. Typical EasySep™ Release Mouse Biotin Positive Selection Kit Cell Isolation Profile from PBMCs

Starting with fresh PBMCs, the purities of the start and final isolated fractions are 34.6% and 97.1%, respectively, using a biotinylated anti-human CD45RO antibody and EasySep™ Release Human Biotin Positive Selection Kit (as assessed by labeling with CD45RO and CD45RA).

Typical EasySep™ Release Mouse Biotin Positive Selection Kit Cell Isolation Profile from Splenocytes

Figure 2. Typical EasySep™ Release Mouse Biotin Positive Selection Kit Cell Isolation Profile from Splenocytes

Starting with mouse splenocytes, the purities of the start and final isolated fractions are 58.4% and 95.1%, respectively, using a biotinylated anti-mouse CD19 antibody and EasySep™ Release Mouse Biotin Positive Selection Kit (as assessed by labeling with CD19 and CD45R/B220).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
17655
Lot #
1000157901 or lower
Language
English
Catalog #
17655
Lot #
1000157902 or higher
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Catalog #
17655
Lot #
All
Language
English

Resources and Publications

Publications (1)

TCF-1 promotes chromatin interactions across topologically associating domains in T cell progenitors. W. Wang et al. Nature immunology 2022 jul

Abstract

The high mobility group (HMG) transcription factor TCF-1 is essential for early T cell development. Although in vitro biochemical assays suggest that HMG proteins can serve as architectural elements in the assembly of higher-order nuclear organization, the contribution of TCF-1 on the control of three-dimensional (3D) genome structures during T cell development remains unknown. Here, we investigated the role of TCF-1 in 3D genome reconfiguration. Using gain- and loss-of-function experiments, we discovered that the co-occupancy of TCF-1 and the architectural protein CTCF altered the structure of topologically associating domains in T cell progenitors, leading to interactions between previously insulated regulatory elements and target genes at late stages of T cell development. The TCF-1-dependent gain in long-range interactions was linked to deposition of active enhancer mark H3K27ac and recruitment of the cohesin-loading factor NIPBL at active enhancers. These data indicate that TCF-1 has a role in controlling global genome organization during T cell development.
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.