EasySep™ Human PE Positive Selection Kit II

Immunomagnetic positive selection kit
Catalog #
17664_C
Immunomagnetic positive selection kit
From: 643 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human PE Positive Selection Kit II is designed to isolate cells that are labeled with PE (phycoerythrin)-conjugated antibodies by positive selection. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

This product replaces the EasySep™ Human PE Positive Selection Kit (Catalog #18551).

For highly purified particle-free cells, we recommend the EasySep™ Release Human PE Positive Selection Kit (Catalog #17654)
Advantages
• Fast and easy-to-use
• No columns required
Components
  • EasySep™ Human PE Positive Selection Kit II (Catalog #17664)
    • EasySep™ PE Selection Cocktail, 1 mL
    • Anti-Human CD32 FcR Blocker, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
  • EasySep™ Human PE Positive Selection Kit II (Catalog #17694)
    • EasySep™ Human PE Positive Selection Kit II (Catalog #17664) x 5
  • RoboSep™ Human PE Positive Selection Kit II (Catalog #17664RF)
    • EasySep™ PE Selection Cocktail, 1 mL
    • Anti-Human CD32 FcR Blocker, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Human
Sample Source
Buffy Coat, Cord Blood, Leukapheresis, Other, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human PE Positive Selection Kit II
Catalog #
17664, 17694
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human PE Positive Selection Kit II
Catalog #
17664RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human PE Positive Selection Kit II
Catalog #
17664
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human PE Positive Selection Kit II
Catalog #
17664
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human PE Positive Selection Kit II
Catalog #
17664
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Human PE Positive Selection Kit II
Catalog #
17664
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human PE Positive Selection Kit II
Catalog #
17664RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human PE Positive Selection Kit II
Catalog #
17664RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human PE Positive Selection Kit II
Catalog #
17664RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Human PE Positive Selection Kit II
Catalog #
17664RF
Lot #
All
Language
English

Educational Materials (7)

Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Data and Publications

Data

Starting with human PBMCs, the purities of the start and final isolated fractions in the above example are 59.1% and 98.7%, respectively, using a PE-conjugated anti-human CD3 antibody and EasySep™ Human PE Positive Selection Kit II.

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