EasySep™ Mouse PE Positive Selection Kit II

Immunomagnetic positive selection kit

More Views

From: 504 USD

Options

* Required Fields

Catalog # (Select a product)
Immunomagnetic positive selection kit
From: 504 USD

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Required Products

Overview

The EasySep™ Mouse PE Positive Selection Kit II is designed to isolate cells that are labeled with PE-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

This kit replaces the EasySep™ Mouse PE Positive Selection Kit (Catalog #18554).

For highly purified particle-free cells, we recommend the EasySep™ Release Mouse PE Positive Selection Kit (Catalog #17656).
Advantages:
• Fast and easy-to-use
• No columns required
Components:
  • EasySep™ Mouse PE Positive Selection Kit II (Catalog #17666)
    • EasySep™ PE Selection Cocktail, 1 mL
    • Mouse FcR Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
  • RoboSep™ Mouse PE Positive Selection Kit II (Catalog #17666RF)
    • EasySep™ PE Selection Cocktail, 1 mL
    • Mouse FcR Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125) x 2
  • EasySep™ Mouse PE Positive Selection Kit II (Catalog #17696)
    • EasySep™ Mouse PE Positive Selection Kit (Catalog #17666) x 5
  • RoboSep™ Mouse PE Positive Selection Kit II (Catalog #17696RF)
    • EasySep™ Mouse PE Positive Selection Kit (Catalog #17666) x 5
    • RoboSep™ Buffer (Catalog #20104) x 5
    • RoboSep™ Filter Tips (Catalog #20125) x 10
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells; Dendritic Cells; Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells; Marrow Stromal Cells; Mesenchymal Stem and Progenitor Cells; Monocytes; Mononuclear Cells; Myeloid Cells; NK Cells; Other; Plasma Cells; T Cells; Macrophages
Species:
Mouse
Sample Source:
Bone Marrow; Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(8)

Data and Publications

Data

Starting with mouse splenocytes, the purities of the start and final isolated fractions in the above example are 20.5% and 91.6%, respectively, using a PEconjugated anti-mouse CD4 antibody and EasySep™ Mouse PE Positive Selection Kit II.

Publications

(1)
Journal of medicinal chemistry 2019 dec

Enzymatic Preparation of 2'-5',3'-5'-Cyclic Dinucleotides, Their Binding Properties to Stimulator of Interferon Genes Adaptor Protein, and Structure/Activity Correlations.

B. Novotn\'a et al.

Abstract

Cyclic dinucleotides are second messengers in the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway, which plays an important role in recognizing tumor cells and viral or bacterial infections. They bind to the STING adaptor protein and trigger expression of cytokines via TANK binding kinase 1 (TBK1)/interferon regulatory factor 3 (IRF3) and inhibitor of nuclear factor-$\kappa$B (I$\kappa$B) kinase (IKK)/nuclear factor-$\kappa$B (NF$\kappa$B) signaling cascades. In this work, we describe an enzymatic preparation of 2'-5',3'-5'-cyclic dinucleotides (2'3'CDNs) with use of cyclic GMP-AMP synthases (cGAS) from human, mouse, and chicken. We profile substrate specificity of these enzymes by employing a small library of nucleotide-5'-triphosphate (NTP) analogues and use them to prepare 33 2'3'CDNs. We also determine affinity of these CDNs to five different STING haplotypes in cell-based and biochemical assays and describe properties needed for their optimal activity toward all STING haplotypes. Next, we study their effect on cytokine and chemokine induction by human peripheral blood mononuclear cells (PBMCs) and evaluate their cytotoxic effect on monocytes. Additionally, we report X-ray crystal structures of two new CDNs bound to STING protein and discuss structure-activity relationship by using quantum and molecular mechanical (QM/MM) computational modeling.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.