Overview
The EasySep™ Release Mouse PE Positive Selection Kit is designed to isolate cells that are labeled with PE-conjugated antibodies from mouse tissue by immunomagnetic positive selection.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, PE-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
This product replaces the EasySep™ Mouse PE Positive Selection Kit (Catalog #18554), providing highly purified particle-free cells.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, PE-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
This product replaces the EasySep™ Mouse PE Positive Selection Kit (Catalog #18554), providing highly purified particle-free cells.
Advantages:
• Highly purified cells labeled with PE-conjugated antibodies isolated from mouse tissue in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components:
- EasySep™ Release Mouse PE Positive Selection Kit (Catalog #17656)
- EasySep™ Release PE Positive Selection Cocktail, 0.5 mL
- EasySep™ Releasable RapidSpheres™ 50201, 1 mL
- EasySep™ Release Buffer (Concentrate), 3 x 1 mL
- Normal Rat Serum, 2 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ Magnet (Catalog #18102)
• EasyEights™ Magnet (Catalog #18103)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells; Dendritic Cells; Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells; Macrophages; Marrow Stromal Cells; Mesenchymal Stem and Progenitor Cells; Monocytes; Mononuclear Cells; Myeloid Cells; NK Cells; Other; Plasma; T Cells
Species:
Mouse
Sample Source:
Bone Marrow; Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
17656
19E102695 or lower
17656
1000005896 and higher
Educational Materials
(8)Data and Publications
Data

Starting with mouse splenocytes, the purities of the start and final isolated fractions are 59.1% and 98.6%, respectively, using a PE-conjugated anti-mouse CD19 antibody and EasySep™ Release Mouse Positive Selection Kit.
Publications
(1)
Journal of medicinal chemistry 2019 dec
Enzymatic Preparation of 2'-5',3'-5'-Cyclic Dinucleotides, Their Binding Properties to Stimulator of Interferon Genes Adaptor Protein, and Structure/Activity Correlations.
Abstract
Abstract
Cyclic dinucleotides are second messengers in the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway, which plays an important role in recognizing tumor cells and viral or bacterial infections. They bind to the STING adaptor protein and trigger expression of cytokines via TANK binding kinase 1 (TBK1)/interferon regulatory factor 3 (IRF3) and inhibitor of nuclear factor-$\kappa$B (I$\kappa$B) kinase (IKK)/nuclear factor-$\kappa$B (NF$\kappa$B) signaling cascades. In this work, we describe an enzymatic preparation of 2'-5',3'-5'-cyclic dinucleotides (2'3'CDNs) with use of cyclic GMP-AMP synthases (cGAS) from human, mouse, and chicken. We profile substrate specificity of these enzymes by employing a small library of nucleotide-5'-triphosphate (NTP) analogues and use them to prepare 33 2'3'CDNs. We also determine affinity of these CDNs to five different STING haplotypes in cell-based and biochemical assays and describe properties needed for their optimal activity toward all STING haplotypes. Next, we study their effect on cytokine and chemokine induction by human peripheral blood mononuclear cells (PBMCs) and evaluate their cytotoxic effect on monocytes. Additionally, we report X-ray crystal structures of two new CDNs bound to STING protein and discuss structure-activity relationship by using quantum and molecular mechanical (QM/MM) computational modeling.
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