EasySep™ Mouse APC Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit

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Immunomagnetic positive selection cell isolation kit
From: 505 USD

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Overview Related Products Scientific Resources Data and Publications

Overview

The EasySep™ Mouse APC Positive Selection Kit II is designed to isolate cells that are labeled with APC (allophycocyanin)-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing APC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

This product replaces the EasySep™ Mouse APC Positive Selection Kit (Catalog #18452).
Advantages:
• Fast and easy-to-use
• No columns required
Components:
  • EasySep™ Mouse APC Positive Selection Kit (Catalog #17667)
    • EasySep™ APC Selection Cocktail, 1 mL
    • Mouse FcR Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
  • RoboSep™ Mouse APC Positive Selection Kit II (Catalog #17667RF)
    • EasySep™ APC Selection Cocktail, 1 mL
    • Mouse FcR Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells; Dendritic Cells; Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells; Marrow Stromal Cells; Mesenchymal Stem and Progenitor Cells; Monocytes; Mononuclear Cells; Myeloid Cells; NK Cells; Other; Plasma Cells; T Cells; Macrophages
Species:
Mouse
Sample Source:
Bone Marrow; Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(7)
Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Human Immune Cytokines
Video
1:57
Fast and Easy Cell Isolation with EasySep™
Video
1:37
How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
Video
1:13
The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells

Data and Publications

Data

Starting with mouse splenocytes, the purities of the start and final isolated fractions in the above example are 19.2% and 99.4% respectively, using an APC-conjugated anti-mouse CD4 antibody and EasySep™ Mouse APC Positive Selection Kit II.

Users of this kit should ensure that they are entitled to use the antibody of interest. STEMCELL Technologies Inc. is not responsible for patent infringements or violations that may occur when using this product.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.