EasySep™ Mouse APC Positive Selection Kit II

Immunomagnetic positive selection of APC-conjugated antibody-labeled mouse cells

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EasySep™ Mouse APC Positive Selection Kit II

Immunomagnetic positive selection of APC-conjugated antibody-labeled mouse cells

From: 625 USD
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Immunomagnetic positive selection of APC-conjugated antibody-labeled mouse cells
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Product Advantages


  • Fast and easy-to-use

  • No columns required

What's Included

  • EasySep™ Mouse APC Positive Selection Kit (Catalog #17667)
    • EasySep™ APC Selection Cocktail, 1 mL
    • Mouse FcR Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
  • RoboSep™ Mouse APC Positive Selection Kit II (Catalog #17667RF)
    • EasySep™ APC Selection Cocktail, 1 mL
    • Mouse FcR Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

Easily isolate highly purified mouse cells labeled with allophycocyanin (APC)-conjugated antibodies from mouse splenocytes, bone marrow, or other single-cell suspensions, using immunomagnetic positive selection, with the EasySep™ Mouse APC Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing APC and magnetic particles. The kit also contains an antibody to mouse Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired cells are ready for downstream applications such as flow cytometry, culture, or cell-based assays.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Starting with mouse splenocytes, the purities of the start and final isolated fractions in the above example are 19.2% and 99.4% respectively, using an APC-conjugated anti-mouse CD4 antibody and EasySep™ Mouse APC Positive Selection Kit II.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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17667RF
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English
Catalog #
17667
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English
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Safety Data Sheet 1
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17667RF
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All
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English
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Safety Data Sheet 2
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17667RF
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All
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English
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Safety Data Sheet 3
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17667RF
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All
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English
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Safety Data Sheet 4
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17667RF
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All
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English
Document Type
Safety Data Sheet 1
Catalog #
17667
Lot #
All
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English
Document Type
Safety Data Sheet 2
Catalog #
17667
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
17667
Lot #
All
Language
English
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more