EasySep™ Mouse APC Positive Selection Kit II
Immunomagnetic positive selection cell isolation kit
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Required Products
-
EasySep™ MagnetMagnet for column-free immunomagnetic separation
-
EasySep™ BufferCell separation buffer
Overview
The EasySep™ Mouse APC Positive Selection Kit II is designed to isolate cells that are labeled with APC (allophycocyanin)-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing APC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
This product replaces the EasySep™ Mouse APC Positive Selection Kit (Catalog #18452).
This product replaces the EasySep™ Mouse APC Positive Selection Kit (Catalog #18452).
Advantages
• Fast and easy-to-use
• No columns required
• No columns required
Components
- EasySep™ Mouse APC Positive Selection Kit (Catalog #17667)
- EasySep™ APC Selection Cocktail, 1 mL
- Mouse FcR Blocker, 0.1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
- RoboSep™ Mouse APC Positive Selection Kit II (Catalog #17667RF)
- EasySep™ APC Selection Cocktail, 1 mL
- Mouse FcR Blocker, 0.1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
Related Products
Related Products
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
EasySep™ Mouse APC Positive Selection Kit II
|
Catalog #
17667 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet
|
Product Name
RoboSep™ Mouse APC Positive Selection Kit II
|
Catalog #
17667RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Mouse APC Positive Selection Kit II
|
Catalog #
17667 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Mouse APC Positive Selection Kit II
|
Catalog #
17667 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
EasySep™ Mouse APC Positive Selection Kit II
|
Catalog #
17667 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
RoboSep™ Mouse APC Positive Selection Kit II
|
Catalog #
17667RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
RoboSep™ Mouse APC Positive Selection Kit II
|
Catalog #
17667RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
RoboSep™ Mouse APC Positive Selection Kit II
|
Catalog #
17667RF |
Lot #
All |
Language
English |
Educational Materials (7)
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Human Immune Cytokines
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Data and Publications
Data
Starting with mouse splenocytes, the purities of the start and final isolated fractions in the above example are 19.2% and 99.4% respectively, using an APC-conjugated anti-mouse CD4 antibody and EasySep™ Mouse APC Positive Selection Kit II.
Legal Statement:
Users of this kit should ensure that they are entitled to use the antibody of interest. STEMCELL Technologies Inc. is not responsible for patent infringements or violations that may occur when using this product.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
