EasySep™ Release Mouse PE Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology
EasySep™ Release Mouse PE Positive Selection Kit

Immunomagnetic positive selection of particle-free mouse cells labeled with PE-conjugated antibodies

1 x 10^9 cells
Catalog # 17656
783 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Release Mouse PE Positive Selection Kit is designed to isolate cells that are labeled with PE-conjugated antibodies from mouse tissue by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, PE-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.

This product replaces the EasySep™ Mouse PE Positive Selection Kit (Catalog #18554), providing highly purified particle-free cells.
Advantages
• Highly purified cells labeled with PE-conjugated antibodies isolated from mouse tissue in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components
  • EasySep™ Release Mouse PE Positive Selection Kit (Catalog #17656)
    • EasySep™ Release PE Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 1 mL
    • EasySep™ Release Buffer (Concentrate), 3 x 1 mL
    • Normal Rat Serum, 2 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ Magnet (Catalog #18102)
• EasyEights™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ Release Mouse PE Positive Selection Kit
Catalog #
17656
Lot #
1000068077 or lower
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Release Mouse PE Positive Selection Kit
Catalog #
17656
Lot #
1000068078 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Release Mouse PE Positive Selection Kit
Catalog #
17656
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Release Mouse PE Positive Selection Kit
Catalog #
17656
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Release Mouse PE Positive Selection Kit
Catalog #
17656
Lot #
All
Language
English

Educational Materials (8)

Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Human Immune Cytokines
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Scientific Poster
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres™

Data and Publications

Data

Starting with mouse splenocytes, the purities of the start and final isolated fractions are 59.1% and 98.6%, respectively, using a PE-conjugated anti-mouse CD19 antibody and EasySep™ Release Mouse Positive Selection Kit.

Publications (1)

Journal of medicinal chemistry 2019 dec Enzymatic Preparation of 2'-5',3'-5'-Cyclic Dinucleotides, Their Binding Properties to Stimulator of Interferon Genes Adaptor Protein, and Structure/Activity Correlations. B. Novotn\'a et al.

Abstract

Cyclic dinucleotides are second messengers in the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway, which plays an important role in recognizing tumor cells and viral or bacterial infections. They bind to the STING adaptor protein and trigger expression of cytokines via TANK binding kinase 1 (TBK1)/interferon regulatory factor 3 (IRF3) and inhibitor of nuclear factor-$\kappa$B (I$\kappa$B) kinase (IKK)/nuclear factor-$\kappa$B (NF$\kappa$B) signaling cascades. In this work, we describe an enzymatic preparation of 2'-5',3'-5'-cyclic dinucleotides (2'3'CDNs) with use of cyclic GMP-AMP synthases (cGAS) from human, mouse, and chicken. We profile substrate specificity of these enzymes by employing a small library of nucleotide-5'-triphosphate (NTP) analogues and use them to prepare 33 2'3'CDNs. We also determine affinity of these CDNs to five different STING haplotypes in cell-based and biochemical assays and describe properties needed for their optimal activity toward all STING haplotypes. Next, we study their effect on cytokine and chemokine induction by human peripheral blood mononuclear cells (PBMCs) and evaluate their cytotoxic effect on monocytes. Additionally, we report X-ray crystal structures of two new CDNs bound to STING protein and discuss structure-activity relationship by using quantum and molecular mechanical (QM/MM) computational modeling.
View All Publications

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