EasySep™ Human Biotin Positive Selection Kit II
Immunomagnetic positive selection cell isolation kit
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Required Products
-
EasySep™ MagnetMagnet for column-free immunomagnetic separation
-
EasySep™ BufferCell separation buffer
Overview
The EasySep™ Human Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
This product replaces the EasySep™ Human Biotin Positive Selection Kit (Catalog #18553).
For highly purified particle-free cells, we recommend the EasySep™ Release Human Biotin Positive Selection Kit (Catalog #17653).
This product replaces the EasySep™ Human Biotin Positive Selection Kit (Catalog #18553).
For highly purified particle-free cells, we recommend the EasySep™ Release Human Biotin Positive Selection Kit (Catalog #17653).
Advantages
• Fast and easy-to-use
• No columns required
• No columns required
Components
- EasySep™ Human Biotin Positive Selection Kit II (Catalog #17663)
- EasySep™ Biotin Selection Cocktail, 1 mL
- Anti-Human CD32 FcR Blocker, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
- RoboSep™ Human Biotin Positive Selection Kit II (Catalog #17663RF)
- EasySep™ Biotin Selection Cocktail, 1 mL
- Anti-Human CD32 FcR Blocker, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Human
Sample Source
Buffy Coat, Cord Blood, Leukapheresis, Other, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
Related Products
Related Products
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
EasySep™ Human Biotin Positive Selection Kit II
|
Catalog #
17663 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet
|
Product Name
RoboSep™ Human Biotin Positive Selection Kit II
|
Catalog #
17663RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Human Biotin Positive Selection Kit II
|
Catalog #
17663 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Human Biotin Positive Selection Kit II
|
Catalog #
17663 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
EasySep™ Human Biotin Positive Selection Kit II
|
Catalog #
17663 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 4
|
Product Name
EasySep™ Human Biotin Positive Selection Kit II
|
Catalog #
17663 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
RoboSep™ Human Biotin Positive Selection Kit II
|
Catalog #
17663RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
RoboSep™ Human Biotin Positive Selection Kit II
|
Catalog #
17663RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
RoboSep™ Human Biotin Positive Selection Kit II
|
Catalog #
17663RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 4
|
Product Name
RoboSep™ Human Biotin Positive Selection Kit II
|
Catalog #
17663RF |
Lot #
All |
Language
English |
Educational Materials (7)
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Data and Publications
Data
Starting with human PBMCs, the purities of the start and final isolated fractions in the above example are 60% and 97.4%, respectively, using a biotinylated anti-human CD3 antibody and EasySep™ Human Biotin Positive Selection Kit II (as assessed using a PE-conjugated anti-human CD3 antibody).
Legal Statement:
Users of this kit should ensure that they are entitled to use the antibody of interest. STEMCELL Technologies Inc. is not responsible for patent infringements or violations that may occur when using this product.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
