EasySep™ Release Mouse APC Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

More Views

EasySep™ Release Mouse APC Positive Selection Kit

Immunomagnetic positive selection of particle-free mouse cells labeled with APC-conjugated antibodies

1 x 109 cells
Catalog #100-0033
731 USD

Required Products

Overview

The EasySep™ Release Mouse APC Positive Selection Kit is designed to isolate cells that are labeled with APC-conjugated antibodies from mouse tissue by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, APC-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Advantages:
• Highly purified cells labeled with APC-conjugated antibodies isolated from mouse tissue in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components:
  • EasySep™ Release Mouse APC Positive Selection Kit (Catalog #100-0033)
    • EasySep™ Release APC Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 1 mL
    • EasySep™ Release Buffer (Concentrate), 3 x 1 mL
    • Normal Rat Serum, 2 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Subtype:
Cell Isolation Kits
Cell Type:
Other
Species:
Mouse
Sample Source:
Bone Marrow; Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(4)

Data and Publications

Data

Using the EasySep™ Release Mouse APC Positive Selection Kit, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.

Figure 1. Purity of CD45+ Cells Following Cell Isolation with the EasySep™ Release Mouse APC Positive Selection Kit

In the above example, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.