EasySep™ Release Human APC Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

EasySep™ Release Human APC Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

EasySep™ Release Human APC Positive Selection Kit
1 x 10^9 cells
746 USD
Catalog # 100-0031

Immunomagnetic positive selection of particle-free human cells labeled with APC-conjugated antibodies

Product Advantages

• Highly purified cells labeled with APC-conjugated antibodies isolated from human samples in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

  • EasySep™ Release Human APC Positive Selection Kit (Catalog #100-0031)
    • EasySep™ Release APC Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 1 mL
    • EasySep™ Release Buffer (Concentrate), 3 x 1 mL
    • Anti-Human CD32 Blocker for Positive Selection, 1 mL

Overview

The EasySep™ Release Human APC Positive Selection Kit is designed to isolate cells that are labeled with APC-conjugated antibodies from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, APC-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Subtype
Cell Isolation Kits
Cell Type
Other
Species
Human
Sample Source
Leukapheresis, Other, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0031
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0031
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0031
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0031
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
100-0031
Lot #
All
Language
English

Data and Publications

Using the EasySep™ Release Human APC Positive Selection Kit, the frequencies of CD45+ cells in the starting and isolated fractions are 5.9% and 94.2%, respectively.

Figure 1. Purity of CD45+ Cells Following Cell Isolation with the EasySep™ Release Human APC Positive Selection Kit

In the above example, the frequencies of CD45+ cells in the starting and isolated fractions are 5.9% and 94.2%, respectively.

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