EasySep™ Release Human APC Positive Selection Kit
Immunomagnetic positive selection kit using particle release technology
Required Products
-
EasySep™ MagnetMagnet for column-free immunomagnetic separation
-
EasySep™ BufferCell separation buffer
Overview
The EasySep™ Release Human APC Positive Selection Kit is designed to isolate cells that are labeled with APC-conjugated antibodies from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, APC-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, APC-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Advantages
• Highly purified cells labeled with APC-conjugated antibodies isolated from human samples in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components
- EasySep™ Release Human APC Positive Selection Kit (Catalog #100-0031)
- EasySep™ Release APC Positive Selection Cocktail, 1 mL
- EasySep™ Releasable RapidSpheres™, 1 mL
- EasySep™ Release Buffer (Concentrate), 3 x 1 mL
- Anti-Human CD32 Blocker for Positive Selection, 1 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Subtype
Cell Isolation Kits
Cell Type
Other
Species
Human
Sample Source
Leukapheresis, Other, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology
Related Products
Related Products
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
EasySep™ Release Human APC Positive Selection Kit
|
Catalog #
100-0031 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Release Human APC Positive Selection Kit
|
Catalog #
100-0031 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Release Human APC Positive Selection Kit
|
Catalog #
100-0031 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
EasySep™ Release Human APC Positive Selection Kit
|
Catalog #
100-0031 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 4
|
Product Name
EasySep™ Release Human APC Positive Selection Kit
|
Catalog #
100-0031 |
Lot #
All |
Language
English |
Educational Materials (8)
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Scientific Poster
Releasable RapidSpheres Enable Immunomagnetic Purification of Highly Viable and Functional Immune Cells from Complex Tissues in Less Than 30 Minutes
Scientific Poster
Fast and Easy Immunomagnetic Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable RapidSpheres™ Results in Functional, Pure and Particle-Free Cells
Scientific Poster
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres™
Data and Publications
Data
Figure 1. Purity of CD45+ Cells Following Cell Isolation with the EasySep™ Release Human APC Positive Selection Kit
In the above example, the frequencies of CD45+ cells in the starting and isolated fractions are 5.9% and 94.2%, respectively.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
