STEMdiff™ Microglia Differentiation Kit

Differentiation kit for the generation of microglia precursors from human ES and iPS cell-derived hematopoietic progenitor cells
STEMdiff™ Microglia Differentiation Kit

Differentiation kit for the generation of microglia precursors from human ES and iPS cell-derived hematopoietic progenitor cells

1 Kit
Catalog # 100-0019
Required Products
  1. STEMdiff™ Hematopoietic Kit
    STEMdiff™ Hematopoietic Kit

    For differentiation of human ES or iPS cells into hematopoietic progenitor cells

  2. DMEM/F-12 with 15 mM HEPES
    DMEM/F-12 with 15 mM HEPES

    Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

  3. Falcon® Conical Tubes, 15 mL
    Falcon® Conical Tubes, 15 mL

    Sterile polypropylene conical tubes

  4. STEMdiff™ Microglia Maturation Kit
    STEMdiff™ Microglia Maturation Kit

    Maturation kit for the generation of microglia from human ES and iPS cell-derived microglia precursors

Overview

The STEMdiff™ Microglia Culture System comprises STEMdiff™ Microglia Differentiation Kit and STEMdiff™ Microglia Maturation Kit. Together, these kits are used to differentiate and mature microglia derived from human pluripotent stem cells (hPSCs) using STEMdiff™ Hematopoietic Kit (Catalog #05310).

Based on the protocol from the laboratory of Mathew Blurton-Jones (Abud et al., 2017), the resulting cells are a highly pure population of microglia (> 80% CD45/CD11b-positive, > 50% TREM2-positive microglia; < 20% morphologically distinct monocytes or macrophages).

Cells derived using these products are versatile tools for modeling neuroinflammation, studying human neurological development and disease, co-culture applications, and toxicity testing.
Advantages
⦁ Greater than 90% of source HPCs are differentiated into microglia.
⦁ Upstream compatibility with HPCs generated using the STEMdiff™ Hematopoietic Kit and co-culture with STEMdiff™ neural organoids.
⦁ Allows differentiation to microglia with few contaminating macrophages or monocytes.
⦁ Microglia generated with the kit demonstrate phagocytosis and activation.
⦁ Required materials are provided in a simple, easy-to-use format.
Components
  • STEMdiff™ Microglia Differentiation Kit (Catalog #100-0019)
    • STEMdiff™ Microglia Basal Medium, 90 mL
    • STEMdiff™ Microglia Supplement 1, 10 mL
    • STEMdiff™ Microglia Supplement 2, 400 uL
Subtype
Specialized Media
Cell Type
Hematopoietic Cells, PSC-Derived, Microglia, Neural Cells, PSC-Derived
Species
Human
Application
Cell Culture, Differentiation
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Immunology, Neuroscience
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Microglia Differentiation Kit
Catalog #
100-0019
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Microglia Differentiation Kit
Catalog #
100-0019
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Microglia Differentiation Kit
Catalog #
100-0019
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
STEMdiff™ Microglia Differentiation Kit
Catalog #
100-0019
Lot #
All
Language
English

Educational Materials (16)

Brochure
STEMdiff™ Hematopoietic Kit
Brochure
STEMCELL Neural Product Portfolio
Brochure
hPSC-Derived Neural Cell Research
Brochure
Products for Human Pluripotent Stem Cells
Protocol
How to Co-Culture Human Pluripotent Stem Cell (hPSC)-Derived Forebrain Neurons and Microglia
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
Video
How to Count hPSC Aggregates to Determine Plating Density for Maintenance and Differentiation
3:39
How to Count hPSC Aggregates to Determine Plating Density for Maintenance and Differentiation
Webinar
ISSCR Innovation Showcase: Advanced Brain Organoid Co-Culture Systems
1:01:37
ISSCR Innovation Showcase: Advanced Brain Organoid Co-Culture Systems
Webinar
Modeling Alzheimer's risk using human TREM2-knockout microglia
56:09
Modeling Alzheimer's risk using human TREM2-knockout microglia
Webinar
STEMdiff™ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
20:24
STEMdiff™ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
Webinar
Modeling Neurodegenerative Diseases with STEMdiff™ Pluripotent Stem Cell-Derived Neural Culture Systems
23:25
Modeling Neurodegenerative Diseases with STEMdiff™ Pluripotent Stem Cell-Derived Neural Culture Systems
Webinar
Engineering the Hematopoietic System to Treat Non-Hematological Disorders
1:03:01
Engineering the Hematopoietic System to Treat Non-Hematological Disorders
Scientific Poster
Using Human Pluripotent Stem Cell-Derived Microglia As Models For Neurological Disease Research
Scientific Poster
Generation of a Glia-Neuron Co-Culture System Derived From Human Pluripotent Stem Cells
Scientific Poster
Generation of Microglia From Human Pluripotent Stem Cells for Neurodegenerative Disease Modeling
Load More Educational Materials

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Experimental protocol schematic for generating microglial precursors from hPSC-derived hematopoietic progenitor cells.

Figure 1. Schematic for the STEMdiff™ Microglia Culture System Protocol

Microglial precursors can be generated in 24 days from hPSC-derived hematopoietic progenitor cells. For the generation of hematopoietic progenitor cells, see documentation for STEMdiff™ Hematopoietic Kit (Catalog #05310). For the maturation of microglial precursors to functional microglia, see the PIS.

RNA-seq profiles of hPSC-derived microglia and other immune cell types are plotted after principal components analysis.

Figure 2. Microglia Generated with STEMdiff™ Microglia Culture System Are Transcriptionally Similar to Those from Published Differentiation and Maturation Protocols

RNA-seq datasets were extracted from 4 different publications (protocols A-D) that generated hPSC-derived microglia and compared their transcriptional profiles to those of other immune cell types (N = 500 genes). Principal component analysis (PCA) was performed on these data along with RNA-seq data from microglia generated with STEMdiff™ Microglia Culture System. The hPSC-derived microglia from STEMdiff™ Microglia Culture System plot most closely to those from protocols A and B.

Time lapse of functional microglia phagocytosing bioindicator particles in culture. Over time, cells turn red and aggregate.

Figure 3. STEMdiff™ Microglia Culture System Generates Functional Microglia Capable of Phagocytosis at Day 34

Microglia taking up pH-sensitive bioindicator particles at a concentration of 250 μg/mL (small dots) were measured over a 12-hour time period with live cell imaging. As the particles are phagocytosed, the cells turn red. Over time, the number of small dots decreased, and the red cells increased in number and aggregated. Scale bar = 100μm.

Figure 4. PSC-Derived Microglia Incorporate into Brain Organoids After 10 Days and Display an Activated Morphology upon Injury.

(A) Representative microglia and brain organoid co-cultures after 10 days, stained with IBA1 for microglia (green) and MAP2 for neurons (magenta). The microglia integrate among the neurons and display an unactivated morphology with extended processes (arrow). (B) The microglia display an activated amoeboid morphology upon injury as shown by IBA1 staining.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.