STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)
Defined animal component-free medium for the differentiation of human ES and iPS cells maintained in TeSR™-E8™ to definitive endoderm
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STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) -
Label for STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) -
Label for STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) -
Label for STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) -
Label for STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)
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Required Products
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RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2 -
Gentle Cell Dissociation Reagent
cGMP, enzyme-free cell dissociation reagent -
Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions
Overview
STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) is a complete, serum- and animal component-free medium and supplement kit that supports highly efficient differentiation of human ES cells and iPS cells that have been maintained in TeSR™-E8™ to definitive endoderm. Cells differentiated using the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) express high levels of endoderm markers, including CD184 (CXCR4) and SOX17. When used in combination with Vitronectin XF™, the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) provides a completely xeno-free workflow for the generation of multipotent definitive endoderm.
Optimized for differentiation of cells maintained in TeSR™-E8™ . For differentiation of cells maintained in mTeSR™1, please see the STEMdiff™ Definitive Endoderm Kit.
Optimized for differentiation of cells maintained in TeSR™-E8™ . For differentiation of cells maintained in mTeSR™1, please see the STEMdiff™ Definitive Endoderm Kit.
Advantages:
• Defined, serum- and animal component-free medium for the differentiation of human ES and iPS cells to definitive endoderm in a complete, ready-to-use format
• Efficient and reproducible differentiation of multiple ES cell and iPS cell lines
• Generates definitive endoderm cells capable of further differentiation to pancreatic, hepatic, intestinal and pulmonary cell lineages
• Optimized for use with cells maintained in TeSR™-E8™ cell culture medium
• Efficient and reproducible differentiation of multiple ES cell and iPS cell lines
• Generates definitive endoderm cells capable of further differentiation to pancreatic, hepatic, intestinal and pulmonary cell lineages
• Optimized for use with cells maintained in TeSR™-E8™ cell culture medium
Components:
- STEMdiff™ Endoderm Basal Medium, 100 mL
- STEMdiff™ Definitive Endoderm Supplement MR (100X), 0.35 mL
- STEMdiff™ Definitive Endoderm Supplement CJ (100X), 1.1 mL
- STEMdiff™ Definitive Endoderm TeSR™-E8™ Supplement, 7 mL
Subtype:
Specialized Media
Cell Type:
Airway Cells; Pluripotent Stem Cells; Hepatic Cells; Intestinal Cells; Pancreatic Cells; Endoderm, PSC-Derived
Species:
Human
Application:
Differentiation; Cell Culture
Brand:
STEMdiff
Area of Interest:
Stem Cell Biology; Epithelial Cell Biology; Cancer Research
Formulation:
Serum-Free; Animal Component-Free
Related Products
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STEMdiff™ Pancreatic Progenitor Kit
Serum-free medium for the differentiation of human ES and iPS cells to pancreatic progenitor cells -
STEMdiff™ Definitive Endoderm Kit
Defined animal component-free medium for the differentiation of human ES and iPS cells to definitive endoderm -
Anti-Human CD184 (CXCR4) Antibody, Clone 12G5
Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD184 (CXCR4)
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
05115
Component #05113: Lot 18D89658 and higher
Educational Materials
(7)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Figure 1. Definitive Endoderm Differentiation is Efficient Across Multiple ES and iPS Cell Lines Maintained in Either mTeSR™1 or TeSR™-E8™ Maintenance Media
Quantitative analysis of definitive endoderm formation in multiple hES (H1 and H9) and hiPS (WLS-4D1 and STiPS-M001) lines as measured by co-expression of CXCR4 and SOX17. Cells maintained in mTeSR™1 or TeSR™-E8™ were differentiated using the STEMdiff™ Definitive Endoderm Kit or the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™-Optimized), respectively. Data are expressed as the mean percentage of cells expressing both markers. Error bars indicate SEM.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.