STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)
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STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) -
Label for STEMdiff™ Definitive Endoderm Kit -
Label for STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)
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Required Products
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Gentle Cell Dissociation Reagent
Enzyme-free cell dissociation reagent -
Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions -
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
Overview
Optimized for differentiation of cells maintained in TeSR™-E8™ . For differentiation of cells maintained in mTeSR™1, please see the STEMdiff™ Definitive Endoderm Kit.
• Efficient and reproducible differentiation of multiple ES cell and iPS cell lines
• Generates definitive endoderm cells capable of further differentiation to pancreatic, hepatic, intestinal and pulmonary cell lineages
• Optimized for use with cells maintained in TeSR™-E8™ cell culture medium
- STEMdiff™ Definitive Endoderm Basal Medium, 100 mL
- STEMdiff™ Definitive Endoderm Supplement A, 0.35 mL
- STEMdiff™ Definitive Endoderm Supplement B, 1.1 mL
- STEMdiff™ Definitive Endoderm TeSR™-E8™ Supplement, 7 mL
Related Products
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STEMdiff™ Definitive Endoderm Kit
Defined animal component-free medium for the differentiation of human ES and iPS cells to definitive endoderm -
Anti-Human CD184 (CXCR4) Antibody, Clone 12G5
Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD184 (CXCR4) -
STEMdiff™ Pancreatic Progenitor Kit
Serum-free, defined medium for the differentiation of human ES and iPS cells to pancreatic progenitor cells
Scientific Resources
Product Documentation
Educational Materials
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. Definitive Endoderm Differentiation is Efficient Across Multiple ES and iPS Cell Lines Maintained in Either mTeSR™1 or TeSR™-E8™ Maintenance Media
Quantitative analysis of definitive endoderm formation in multiple hES (H1 and H9) and hiPS (WLS-4D1 and STiPS-M001) lines as measured by co-expression of CXCR4 and SOX17. Cells maintained in mTeSR™1 or TeSR™-E8™ were differentiated using the STEMdiff™ Definitive Endoderm Kit or the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™-Optimized), respectively. Data are expressed as the mean percentage of cells expressing both markers. Error bars indicate SEM.