Healthy Control Human iPSC Line, Female, SCTi005-A

Human pluripotent stem cell line, frozen

Healthy Control Human iPSC Line, Female, SCTi005-A

Human pluripotent stem cell line, frozen

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Human pluripotent stem cell line, frozen
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Product Advantages

  • Enable academic and commercial research with ethically sourced human iPSCs collected using IRB protocols
  • Trust in extensive quality control that meets or exceeds industry standards at every step of the manufacturing process (ISCBI, 2009 and ISSCR Standards, 2023)
  • Enhance research transparency and ethical and biological conformity by using a cell line verified by hPSCreg®
  • Confidently integrate human iPSCs into your workflow with a cell line compatible with TeSR™ media for maintenance and STEMdiff™ for differentiation

What's Included

  • Healthy Control Human iPSC Line, Female, SCTi005-A (Catalog #200-0944)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

Start your research confidently with a reliable source of high-quality induced pluripotent stem cells (iPSCs) from the SCTi005-A cell line. SCTi005-A is derived from healthy female donor peripheral blood mononuclear cells (PBMCs), making it suitable for various applications, such as for use as an unaffected control, gene editing, or downstream differentiation to lineage-specific cell types and organoids.

To ensure optimal product performance and reproducibility, SCTi005-A is manufactured using extensive quality control procedures in a culture system consisting of mTeSR™ Plus, Corning® Matrigel® hESC-Qualified Matrix, and ReLeSR™. SCTi005-A is karyotypically stable, demonstrates trilineage differentiation potential, expresses undifferentiated cell markers, and was reprogrammed using a non-integrating reprogramming technology. Registration with hPSCreg® ensures ethical and biological conformity based on community standards.

Choose TeSR™ and STEMdiff™ cell culture media products to establish a complete workflow for your cell culture system. For alternative genotypes for your iPSC control lines, see all of our high-quality, healthy control human iPSC lines, female and male.

This research-use-only (RUO) product has been consented for both academic and commercial use. Blood samples are ethically sourced using Institutional Review Board (IRB) or other regulatory authority-approved consent forms and protocols. For donor details and cell quality characterization of the source cell banks, refer to the data figures on this page. For additional details, refer to the lot-specific Certificate of Analysis and Frequently Asked Questions about induced pluripotent stem cell lines.

Whole exome and whole genome sequence data files are available upon request. Please contact us for pricing.

Certain products are only available in select territories. Please contact your sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.
Contains

CryoStor® CS10

Subtype
Frozen
Cell Type
Pluripotent Stem Cells
Species
Human
Cell and Tissue Source
Peripheral Blood, Pluripotent Stem Cells
Application
Cell Culture, Differentiation, Expansion, Genome Editing
Area of Interest
Cell Line Development, Disease Modeling, Drug Discovery and Toxicity Testing, Infectious Diseases, Neuroscience, Organoids, Stem Cell Biology
Donor Status
Normal

Data Figures

Table 1. iPSC Line SCTi005-A Is Derived from a Healthy Female Donor

Table 1. iPSC Line SCTi005-A Is Derived from a Healthy Female Donor

Demographic, health, and genetic characteristics of the SCTi005-A donor are compiled based on self-reported information and whole-exome sequencing. Sex was determined by karyotype. Ancestry and HLA haplotype were calculated from whole-genome and whole-exome sequencing combined data. Blood type (ABO/Rh blood group) was determined by next-generation sequencing. Height, weight, and BMI were calculated at the donation facility. iPSC = induced pluripotent stem cell.

SCTi005-A Human Pluripotent Stem Cells Demonstrate High-Quality Morphology in Routine Culture

Figure 1. SCTi005-A Human Pluripotent Stem Cells Demonstrate High-Quality Morphology in Routine Culture

Cryopreserved cells from line SCTi005-A were thawed and maintained in mTeSR™ Plus on Corning® Matrigel® Matrix.

(A) The resulting iPSC colonies have densely packed cells and show multi-layering when ready to be passaged.

(B,C) Cells retain prominent nucleoli and high nuclear-to-cytoplasmic ratios. iPSC = induced pluripotent stem cell.

Figure 2. SCTi005-A Human Pluripotent Stem Cells Maintain a Normal Karyotype

Figure 2. SCTi005-A Human Pluripotent Stem Cells Maintain a Normal Karyotype

(A) G-T-L banding for thawed cells at p12 (n = 20) shows a normal karyotype with no evidence of clonal abnormalities at a band resolution of 425 - 500 G-bands per haploid genome.

(B) Fluorescent in situ hybridization in a representative p12 iPSC using probes for 20p11 (green) and 20q11.21 (red). 95% of cells examined displayed two sets of two probe signals, indicating no aneusomy of chromosome 20 (n = 200). iPSC = induced pluripotent stem cell.

Table 2. Single Nucleotide Polymorphism Microarray Analysis Characterizes SCTi005-A Copy Number Variants

Table 2. Single Nucleotide Polymorphism Microarray Analysis Characterizes SCTi005-A Copy Number Variants

DNA was extracted from a vial of SCTi005-A from the Master Cell Bank and subject to SNP microarray analysis to identify large-scale copy number variants (CNVs). The cells display no reportable CNVs, defined as those greater than 400kb in size. Array design, genomics position, genes, and chromosome banding are based on genome build GRCh38/hg3. chr = chromosome; bp = base pairs; SNP = single nucleotide polymorphism.

Figure 3. SCTi005-A iPSCs Express Undifferentiated Cell Markers

Figure 3. SCTi005-A iPSCs Express Undifferentiated Cell Markers

Cell line SCTi005-A was characterized using flow cytometry for undifferentiated cell markers OCT3/4 and TRA-1-60.

(A) Percentage marker expression was quantified 3 passages after thawing from the analyses of two biological replicates.

Representative flow cytometry plots are displayed for (B) OCT3/4 and (C) TRA-1-60. iPSC = induced pluripotent stem cell.

Figure 4. SCTi005-A Human Pluripotent Stem Cells Demonstrate a High Trilineage Differentiation Capacity

Figure 4. SCTi005-A Human Pluripotent Stem Cells Demonstrate a High Trilineage Differentiation Capacity

Cells from SCTi005-A were split into 3 groups, differentiated using STEMdiff™ Trilineage Differentiation Kit (Catalog #05230), and then subjected to flow cytometry analysis. Two markers for each embryonic germ layer were assessed, and bars present mean marker expression for each group of cells (n = 2 biological replicates). PAX6 and Nestin confirm differentiation to the ectoderm lineage, NCAM and Brachyury (T) to the mesoderm lineage, and CXCR4 and SOX17 to the endoderm lineage.

Table 3. Comprehensive Risk Variant Profile for the SCTi005-A iPSC Line

Table 3. Comprehensive Risk Variant Profile for the SCTi005-A iPSC Line

The table presents risk variants identified for the SCTi005-A iPSC line. DNA was purified from the master cell bank, and whole-genome sequencing was performed to capture the entire genomic landscape. Sequencing was conducted on the NovaSeq 6000 System, achieving a 50x coverage. Coverage of coding regions and adjacent splice junction sites was enhanced for 20,000 genes using the SureSelect Human All Exon V6 Kit (Agilent Technologies). Variants were called and analyzed following GATK4 best practices, incorporating a Convolutional Neural Network to score each variant. High-confidence variants were annotated with predicted functional consequences and ClinVar IDs. ClinVar entries required two or more supporting ALT reads and an assertion criteria of one gold star or more in ClinVar to be considered. This comprehensive profile assists in understanding the genetic landscape and potential health impacts associated with the SCTi005-A iPSC line.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
200-0944
Lot #
All
Language
English

Resources and Publications

Educational Materials (30)