STEMdiff™

Human pluripotent stem cell (hPSC)-based models hold tremendous potential for the study of human development and disease. When working with hPSCs, even the most detailed and rigorously-followed protocols can still lead to inconsistent differentiation.1,2 Use STEMdiff™ to reproducibly differentiate across multiple human embryonic stem (ES) cell and induced pluripotent stem (iPS) cell lines. STEMdiff™ is part of the most complete system of reagents for human pluripotent stem cell (hPSC) culture - a system that includes mTeSR™1, the most published feeder-free ES cell and iPS cell culture medium. Media formulations, raw material specifications and manufacturing processes for all STEMdiff™ products have been carefully optimized in order to minimize reagent variability. In addition, STEMdiff™ products include detailed, user-friendly protocols to standardize your differentiation procedures.

Why Use STEMdiff™?

  • Reduce variability between experiments.
  • Use with hPSCs cultured with TeSR™ maintenance media.
  • Optimized formulations and pre-screened components ensure reagent consistency and minimal lot-to-lot variability.
  • Detailed protocols for differentiating to all three germ layers.
  • Efficient differentiation of multiple human ES and iPS cell lines.

Which Lineages Are You Interested in Differentiating to?

Ectodermal Lineages

Protocol Type:

Features/Advantages:

  • Rapid neural induction using EB or monolayer protocols
  • Serum-free* expansion and cryopreservation of neural progenitor cells
  • Highly efficient generation of neuronal precursors from neural progenitor cells
  • Maturation of precursors to functional forebrain-type neurons or cerebral organoids

Mesodermal Lineages

Protocol Type:

Monolayer

Features/Advantages:

  • Rapid mesodermal induction after only 2 - 4 days of differentiation
  • Generate multipotent early mesoderm cells that can be further differentiated to multiple downstream mesodermal cell lineages including endothelial, osteogenic, chondrocytes and adipocytes

Endodermal Lineages

Protocol Type:

Monolayer

Features/Advantages:

  • Generate multipotent definitive endoderm cells that can be differentiated to multiple downstream endodermal cell lineages including hepatic, pancreatic, intestinal and pulmonary
  • Generate functional pancreatic progenitor cells that can be further differentiated to insulin-producing β-cells
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Flexible User-Directed Differentiation

For customized differentiation protocols, explore a range of unsupplemented media that supports cell survival without lineage bias. Use to direct differentiation to a variety of cell lineages, including endothelial,3 smooth muscle,3 hematopoietic,4,5 skeletal muscle,6 and cardiomyocytes7.

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