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hPSC Genetic Analysis Kit

qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

hPSC Genetic Analysis Kit

qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells

From: 764 USD
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qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells
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What's Included

  • qPCR Master Mix (2X), 3 mL
  • ROX Reference Dye, 0.2 mL
  • Chr 1q Genetic Assay, 60 Rxn
  • Chr 4p Genetic Assay, 60 Rxn
  • Chr 8q Genetic Assay, 60 Rxn
  • Chr 10p Genetic Assay, 60 Rxn
  • Chr 12p Genetic Assay, 60 Rxn
  • Chr 17q Genetic Assay, 60 Rxn
  • Chr 18q Genetic Assay, 60 Rxn
  • Chr 20q Genetic Assay, 60 Rxn
  • Chr Xp Genetic Assay, 60 Rxn
  • Genomic DNA Control, 15 µL
  • TE Buffer, 1 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Axygen® Sealing Film for Quantitative Real-Time PCR (qPCR)
    Axygen® Sealing Film for Quantitative Real-T...

    Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR)

  2. Genomic DNA Purification Kit
    Genomic DNA Purification Kit

    For purification of genomic DNA from cells or tissue

Overview

Quickly and cost-effectively detect the 8 most common karyotypic abnormalities reported in human pluripotent stem cells (hPSCs).

This qPCR-based kit enables the genetic screening of multiple hPSC lines and contains enough material to analyze 20 individual samples in triplicate. It uses double-quenched probes with a 5-carboxyfluorescein (5-FAM) dye to give superior performance over other, single-quenched probes. The kit includes qPCR Master Mix, ROX Reference Dye, Buffer, and primer/probe mixes designed to detect the critical minimal regions of the 8 most commonly mutated regions, as well as a primer/probe mix to a control location in the genome. A genomic DNA control is also provided with demonstrated normal copy number over the regions of interest.

To understand your results, input qPCR data into our online Genetic Analysis App, which will perform statistical analyses, assist with data interpretation, and provide visual representation of the data
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Genome Editing
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology

Data Figures

Figure 1. The hPSC Genetic Analysis Kit Identifies Chromosome 12 Trisomy

Chromosome 12 trisomy in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.

Figure 2. The hPSC Genetic Analysis Kit Identifies Chromosome 1 Duplication via Unbalanced Translocation

Unbalanced rearrangement of chromosome 1 in the WLS-1C human iPS cell line in which an extra copy of the long (q) arm of chromosome 1 translocated to the short arm (p) of chromosome 21 was (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.

Figure 3. The hPSC Genetic Analysis Kit Identifies Chromosome 20q11.21 Duplication

Chromosome 20q duplication in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar), (B) undetected by G-banding, and (C) confirmed by fluorescent in situ hybridization using probes for 20p11 (green) and 20q11.21 (red).

Figure 4. The hPSC Genetic Analysis Kit Identifies Abnormalities in Cultures with Approximately 30% Mosaicism

Genetically normal WLS-1C human iPS cells were mixed in the indicated ratios with WLS-1C human iPS cells containing a chromosome 20q duplication. Cultures with approximately 30% genetically abnormal cells exhibit a significantly enriched population of 20q11.21 duplication (orange bars).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
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Document Type
Product Information Sheet
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
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Safety Data Sheet 2
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
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English
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Safety Data Sheet 3
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
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All
Language
English
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Safety Data Sheet 4
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
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All
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English
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Safety Data Sheet 5
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
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English
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Safety Data Sheet 6
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
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All
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English
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Safety Data Sheet 7
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
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English
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Safety Data Sheet 8
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
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All
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English
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Safety Data Sheet 9
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
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English
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Safety Data Sheet 10
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
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English
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Safety Data Sheet 11
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
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English
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Safety Data Sheet 12
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English
Document Type
Safety Data Sheet 13
Product Name
hPSC Genetic Analysis Kit
Catalog #
07550
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (36)

Detect Karyotypic Abnormalities in Human ES & iPS Cells
Brochure
Detect Karyotypic Abnormalities in Human ES & iPS Cells
hPSC-Derived Cell Therapy Workflow
Brochure
hPSC-Derived Cell Therapy Workflow
hPSC Genetic Analysis Kit Technical Tips & Tricks
Technical Bulletin
hPSC Genetic Analysis Kit Technical Tips & Tricks
Derivation and Applications of Human Pluripotent Stem Cells
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
7:57
Video
How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
3:35
Video
How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
How to Transition Human Pluripotent Stem Cells into mTeSR™ Plus from Other Feeder-Free Media
1:29
Video
How to Transition Human Pluripotent Stem Cells into mTeSR™ Plus from Other Feeder-Free Media
How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus
5:20
Video
How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus
How to Set Up an Assay with the hPSC Genetic Analysis Kit Experiment
8:33
Video
How to Set Up an Assay with the hPSC Genetic Analysis Kit Experiment
Nature Research Round Table: Pluripotency Tests
17:46
Webinar
Nature Research Round Table: Pluripotency Tests
Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells
24:46
Webinar
Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells
Enhancing Genetic Stability in Human Pluripotent Stem Cells Maintained As Single Cells: Introducing eTeSR™
33:00
Webinar
Enhancing Genetic Stability in Human Pluripotent Stem Cells Maintained As Single Cells: Introducing ...
Re-Creating Disease with Kidney Organoids and CRISPR
1:14:36
Webinar
Re-Creating Disease with Kidney Organoids and CRISPR
Survey Results: Insights and Trends in Pluripotent Stem Cell Research
13:58
Webinar
Survey Results: Insights and Trends in Pluripotent Stem Cell Research
Nature Research Round Table: Genomic Integrity of Human Pluripotent Stem Cells
15:47
Webinar
Nature Research Round Table: Genomic Integrity of Human Pluripotent Stem Cells
Quality Control Guidelines for Clinical-Grade Human Induced Pluripotent Stem Cell Lines
1:13:44
Webinar
Quality Control Guidelines for Clinical-Grade Human Induced Pluripotent Stem Cell Lines
Nature Research Round Table: Identifying Acquired and Background Genetic Variants in Human Pluripotent Stem Cells
16:56
Webinar
Nature Research Round Table: Identifying Acquired and Background Genetic Variants in Human Pluripote...
Nature Research Round Table: Regulations Around Human Induced Pluripotent Stem Cell Registration
21:35
Webinar
Nature Research Round Table: Regulations Around Human Induced Pluripotent Stem Cell Registration
CRISPR-Cas9 Gene Editing of Cerebral Organoids to Model Microcephaly
10:08
Webinar
CRISPR-Cas9 Gene Editing of Cerebral Organoids to Model Microcephaly
Nature Research Round Table: Human Pluripotent Stem Cell Lines as Disease Models
17:30
Webinar
Nature Research Round Table: Human Pluripotent Stem Cell Lines as Disease Models
Consequences of Culture-Acquired Genetic Changes in Human Pluripotent Stem Cells
57:26
Webinar
Consequences of Culture-Acquired Genetic Changes in Human Pluripotent Stem Cells
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
50:49
Webinar
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
From hPSCs to Immune Cells: Mechanisms of Selection in Human Pluripotent Stem Cell Cultures
28:31
Webinar
From hPSCs to Immune Cells: Mechanisms of Selection in Human Pluripotent Stem Cell Cultures
Maintaining and Assessing High-Quality hPSC Cultures
55:53
Webinar
Maintaining and Assessing High-Quality hPSC Cultures
Nature Research Round Table: Best Practices for the QC of Genome-Edited hPSC Lines - Panel Discussion
31:25
Webinar
Nature Research Round Table: Best Practices for the QC of Genome-Edited hPSC Lines - Panel Discussio...
Human Pluripotent Stem Cells for the Treatment of Age-Related Macular Degeneration and Compliance Considerations for Clinical-Grade iPSCs
47:23
Webinar
Human Pluripotent Stem Cells for the Treatment of Age-Related Macular Degeneration and Compliance Co...
Nature Research Round Table: Genome Editing in Human Pluripotent Stem Cells
17:45
Webinar
Nature Research Round Table: Genome Editing in Human Pluripotent Stem Cells
Improving Reproducibility of Your hPSC Research by Generating a High-Quality Cell Bank
21:53
Webinar
Improving Reproducibility of Your hPSC Research by Generating a High-Quality Cell Bank
Nature Research Round Table: HLA Typing Considerations for Human Pluripotent Stem Cell Banking
13:01
Webinar
Nature Research Round Table: HLA Typing Considerations for Human Pluripotent Stem Cell Banking
Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenance of Human Pluripotent Stem Cells (hPSCs)
42:10
Webinar
Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenanc...
Nature Research Round Table: hPSC Lines for Cell Therapies - Panel Discussion
29:03
Webinar
Nature Research Round Table: hPSC Lines for Cell Therapies - Panel Discussion
Comparative Study: Improving the Genetic Stability of Single-Cell-Passaged Human Pluripotent Stem Cell Cultures Using eTeSR™
22:32
Webinar
Comparative Study: Improving the Genetic Stability of Single-Cell-Passaged Human Pluripotent Stem Ce...
Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells
19:35
Webinar
Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells
Nature Research Round Table: The Process of Human Pluripotent Stem Cell Adaptation
18:48
Webinar
Nature Research Round Table: The Process of Human Pluripotent Stem Cell Adaptation
Considerations for High-Efficiency Genome Editing of Human Pluripotent Stem Cells
50:01
Webinar
Considerations for High-Efficiency Genome Editing of Human Pluripotent Stem Cells
Nature Research Round Table: Standards for Pluripotent Stem Cell Banking
13:02
Webinar
Nature Research Round Table: Standards for Pluripotent Stem Cell Banking

Related Products

Related Products
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

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